Expression of FasL gene in porcine chondrocytes

Residential College	false
Status	已發表Published
	Expression of FasL gene in porcine chondrocytes
	Wang S.J.; Wang Y.; Zhang H.Z.; Jin S.; Shen T.W.; Ge Y.; Ge H.L.
	2005-03-01
Source Publication	Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
ISSN	10078738
Volume	21 Issue:2 Pages:133-136
Abstract	AIM: To express the Fas ligand(FasL) gene in porcine chondrocytes. METHODS: The porcine FasL gene fragment was amplified by RT-PCR and then inserted into the pGCEN retroviral vector. The recombinant vector was transfected into packaging cells PA317 which were then screened with G418. Supernatant of screened PA317 cells containing high titer recombinant virus was used to infect porcine chondrocytes. Expression of FasL in chondrocytes was analyzed by FACS and Western blot. RESULTS: The recombinant pGCEN-FasL retroviral expression vector was successfully constructed as shown by restriction enzyme digestion analysis and DNA sequencing. FasL was expressed in 57% of infected chondrocytes. Western blot analysis also confirmed the expression of FasL in chondrocytes. The expressed FasL could induce apoptosis of Fas+ cells. CONCLUSION: The recombinant pGCEN-FasL retroviral vector has been successfully constructed and FasL with biological activity was highly expressed in porcine chondrocytes, which lays the foundation for allogenic chondrocytes transplantation.
URL	View the original
Language	英語English
Fulltext Access	View Full-Text via Scopus
Document Type	Journal article
Collection	University of Macau
Affiliation	Shanghai Second Medical University
Recommended Citation GB/T 7714	Wang S.J.,Wang Y.,Zhang H.Z.,et al. Expression of FasL gene in porcine chondrocytes[J]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 2005, 21(2), 133-136.
APA	Wang S.J.., Wang Y.., Zhang H.Z.., Jin S.., Shen T.W.., Ge Y.., & Ge H.L. (2005). Expression of FasL gene in porcine chondrocytes. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 21(2), 133-136.
MLA	Wang S.J.,et al."Expression of FasL gene in porcine chondrocytes".Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 21.2(2005):133-136.

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