The effect of esterases on 17α-hydroxyprogesterone caproate

doi:10.1016/j.ajog.2007.07.038

UM > Institute of Chinese Medical Sciences

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	The effect of esterases on 17α-hydroxyprogesterone caproate
	Ru Yan; Valentina Fokina; Gary D. V. Hankins; Mahmoud S. Ahmed; Tatiana N. Nanovskaya
	2008-02
Source Publication	AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
ISSN	0002-9378
Volume	198 Issue:2 Pages:229.e1-229.e5
Abstract	Objective The aim of this investigation is to determine whether 17α-hydroxyprogesterone caproate is hydrolyzed, in vitro, to 17α-hydroxyprogesterone and caproate. Study Design The in vitro hydrolysis of dual radioactively labeled 17α-hydroxy-[³H] progesterone [¹⁴C] caproate by human plasma, hepatic and placental S9 fractions as well as recombinant esterases was investigated. The formation of [³H]-17α-hydroxyprogesterone and [¹⁴C]-caproate were determined with the use of high-performance liquid chromatography equipped with an online radioactivity detector. The presence and activity of carboxylesterase and butyrylcholinesterase in the human-derived preparations was confirmed by the hydrolysis of their prototypic substrates p-nitrophenyl acetate, p-nitrophenyl butyrate, and butyrylthiocholine, respectively. Results The aforementioned human-derived preparations hydrolyzed p-nitrophenyl acetate, p-nitrophenyl butyrate, and butyrylthiocholine. However, when 17α-hydroxyprogesterone caproate was incubated with the human-derived preparations under identical experimental conditions neither [³H]-17α-hydroxyprogesterone nor [¹⁴C]-caproate was detected. Conclusion 17α-hydroxyprogesterone caproate is not hydrolyzed in vitro by the esterase enzymes present in human plasma, liver, preterm, or term placenta.
Other Abstract
Keyword	17α-hydroxyprogesterone Caproate Hydrolysis Spontaneous Preterm Labor
DOI	10.1016/j.ajog.2007.07.038
Indexed By	SCIE
Language	英語English
WOS Research Area	Obstetrics & Gynecology
WOS Subject	Obstetrics & Gynecology
WOS ID	WOS:000253587300032
Scopus ID	2-s2.0-38349136867
Fulltext Access	View Full-Text via DOI View Full-Text via Web of Science
Citation statistics
Document Type	Journal article
Collection	Institute of Chinese Medical Sciences
Affiliation	Department of Obstetrics & Gynecology, University of Texas Medical Branch, Galveston, TX.
Recommended Citation GB/T 7714	Ru Yan,Valentina Fokina,Gary D. V. Hankins,et al. The effect of esterases on 17α-hydroxyprogesterone caproate[J]. AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2008, 198(2), 229.e1-229.e5.
APA	Ru Yan., Valentina Fokina., Gary D. V. Hankins., Mahmoud S. Ahmed., & Tatiana N. Nanovskaya (2008). The effect of esterases on 17α-hydroxyprogesterone caproate. AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 198(2), 229.e1-229.e5.
MLA	Ru Yan,et al."The effect of esterases on 17α-hydroxyprogesterone caproate".AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY 198.2(2008):229.e1-229.e5.

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Objective

Study Design

Results

Conclusion