Genome-wide characterization of transcription regulation in fungi

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Status	已發表Published
	Genome-wide characterization of transcription regulation in fungi
	Wong, K. H.; Tan, K.; Hynes, M; Struhl, K
	2014-09-01
Source Publication	Frontiers in Fungal Systems Biology
Abstract	Chromatin Immuno-precipitation (ChIP) is a powerful technique for measuring protein-DNA interactions. When coupled with the Next Generation Sequencing (seq) technology, ChIP-seq can map whole-genome locations and occupancies of any protein-of-interest at extremely high resolution. ChIP-seq has been an indispensable technique in chromatin and transcriptional regulation studies, but this powerful technique has not yet been widely applied in fungi. The output of current sequencing technologies vastly exceeds the sequencing depth requirement of fungal ChIP-seq experiments and therefore a high level of multiplexing can be carried out in a single sequencing run, making this genome-wide profiling method greatly affordable. We have extensively applied ChIP-seq to study different aspects of transcription regulation in Saccharomyces cerevisiae and Aspergillus nidulans. Examples on how ChIP-seq can be broadly applied to address different questions related to genome-wide transcription and chromatin regulations will be presented.
Keyword	Genomics Transcription regulation Fungi
URL	View the original
Language	英語English
The Source to Article	PB_Publication
PUB ID	13519
Document Type	Conference paper
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES Faculty of Health Sciences
Corresponding Author	Wong, K. H.
Recommended Citation GB/T 7714	Wong, K. H.,Tan, K.,Hynes, M,et al. Genome-wide characterization of transcription regulation in fungi[C], 2014.
APA	Wong, K. H.., Tan, K.., Hynes, M., & Struhl, K (2014). Genome-wide characterization of transcription regulation in fungi. Frontiers in Fungal Systems Biology.

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