Comparative genomics study between wild type diploid and avirulent haploid Candida albicans strains to identify virulence factors

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	Comparative genomics study between wild type diploid and avirulent haploid Candida albicans strains to identify virulence factors
	Pardeshi, L.; Zeng, G.; Wang, F.; Wang, H.; Wang, Y.; Wong, K. H.
	2017-06-21
Source Publication	4th Macau Symposium on Biomedical Sciences 2017
Abstract	Candida albicans was regarded as obligate diploid organism until Hickman et al discovered haploid strains among clinical isolates. The haploid strains show many similar characteristic phenotypes like yeast-hyphae transition, white-opaque switching and chlamydospore formation as diploid wild type strains. Studies involving haploid strains to understand the biological phenomenon in diploid C. albicans are on rise, because with only a single set of chromosomes, it is easy to apply various genetic tools on the haploid strains to study these processes. However, the haploids also have some key difference from the wild type. In particular, the haploids are slower in growth rate and have lost virulence compared to diploid C. albicans. Therefore, these avirulent haploid strains also offer an exciting opportunity to dissect the genetic basis of fungal virulence. With the Next Generation Sequencing technology, it is now readily feasible to perform comparative study of any organisms and species at very high resolution. In this study, we have performed whole genome sequencing of two avirulent haploid strains GZY792 and GZY892 and diploid wild type laboratory (SC5314) strains from various labs. We assembled the genome of these strains independently and have identified key differences among the strains. We have also determined the allele selection in each of the haploid strain at nucleotide level resolution. With all this information, we aim to explain possible genetic features for the loss of virulence in the haploid C. albicans strains. This comparative genomics study will help the community to understand and explain the differences between haploid and diploid strains and provide critical useful information for using the haploid strains in genetic studies of C. albicans.
Keyword	Candida albicans haploid diploid
Language	英語English
The Source to Article	PB_Publication
PUB ID	32292
Document Type	Conference paper
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES Faculty of Health Sciences
Corresponding Author	Wong, K. H.
Recommended Citation GB/T 7714	Pardeshi, L.,Zeng, G.,Wang, F.,et al. Comparative genomics study between wild type diploid and avirulent haploid Candida albicans strains to identify virulence factors[C], 2017.
APA	Pardeshi, L.., Zeng, G.., Wang, F.., Wang, H.., Wang, Y.., & Wong, K. H. (2017). Comparative genomics study between wild type diploid and avirulent haploid Candida albicans strains to identify virulence factors. 4th Macau Symposium on Biomedical Sciences 2017.

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