| Status | 已發表Published |
| A protease gain from insect via Horizontal Gene Transfer plays regulatory roles in Aspergillus nidulans nitrogen metabolism. | |
Li, A. ; Wong, K. H. 
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| 2017-06-21 | |
| Source Publication | 4th Macau Symposium on Biomedical Sciences 2017
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| Abstract | Nitrogen metabolism is tightly controlled by a conserved regulatory mechanism called Nitrogen metabolite repression in fungi. In Aspergillus nidulans, expression of nitrogen utilization genes is activated under starvation conditions by the global transcription factor AreA, while AreA activity under nitrogen sufficient conditions is negatively controlled by NmrA, a co-repressor of AreA. The highly expression of NmrA under nitrogen sufficient condition ensures the priority of utilization of preferred nitrogen sources. It has been shown that NmrA protein level drops down under nitrogen starvation conditions. A previous in vitro study found three proteins have the ability to cleave NmrA and identified the AN2366 gene encoding one of them. However, the role of AN2366 has not been confirmed in vivo and the other two proteases are still unknown. In this study, we use a Functional Genomics approach to systematically identify the candidate proteases involved in regulating NmrA turnover. We found that AreA activates the expression of AN2366 under nitrogen starvation conditions, and consequently, AN2366 increases the degradation of NmrA. Therefore, AreA controls its own regulatory capacity by controlling a regulatory loop of nitrogen starvation response. Interestingly, AreA and NmrA are conserved in filamentous fungi, while AN2366 only exist in 5 filamentous fungi and has high identity with insect trypsin like proteins. We propose that it is a product of horizontal gene transfer (HGT) from insect. The HGT event happened from ancient insect to the ancient of these filamentous fungi. From whole genome BLASTp, we aim to found other genes involved in this HGT event. |
| Keyword | Nitrogen protease |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 32297 |
| Document Type | Conference paper |
| Collection | DEPARTMENT OF BIOMEDICAL SCIENCES Faculty of Health Sciences |
| Corresponding Author | Wong, K. H. |
| Recommended Citation GB/T 7714 | Li, A.,Wong, K. H.. A protease gain from insect via Horizontal Gene Transfer plays regulatory roles in Aspergillus nidulans nitrogen metabolism.[C], 2017. |
| APA | Li, A.., & Wong, K. H. (2017). A protease gain from insect via Horizontal Gene Transfer plays regulatory roles in Aspergillus nidulans nitrogen metabolism.. 4th Macau Symposium on Biomedical Sciences 2017. |
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