Garcinone E exerts anticancer properties on ovarian cancer cells

UM > Institute of Chinese Medical Sciences

Status	已發表Published
	Garcinone E exerts anticancer properties on ovarian cancer cells
	Xu, XH; Li, T; Zhang, LL; Chen, X; Guo, X; Chen, X. P.; Wang, Y. T.; Lin, L.; Lu, J.
	2016-08-01
Source Publication	15th Meeting of Consortium for Globalization of Chinese Medicine
Abstract	Garcinone E, a xanthone derivative isolated from mangosteen, has been reported to have cytotoxic capacities. Our recent study suggested that garcinone E exhibits anticancer effects on ovarian cancer cell lines. MTT assay, LDH release assay, Hoechst 33342 staining, Annexin V/PI staining, and JC-1 staining were used to test the antitumor abilities on several ovarian cancer cell lines, and wound-healing assay, transwell assay, and gelatin zymography were carried out to examine the migratory and invasive effects of malignant ovarian cancer HEY cells. Relevant mRNA transcription and protein expression were evaluated by qPCR and western blotting, respectively. Garcinone E treatment significantly inhibited the proliferation and caused cell death on ovarian cancer HEY, A2780 as well as A2780-palicitaxel resistant cells. It induced endoplasmic reticulum stress in HEY cells and activated IRE signaling pathway, which provided protection on HEY cells. Meanwhile, knocking down of IRE-1α by siRNA further activated caspase cascade and caused more cell death. On the other hand, garcinone E eliminated migrative ability of HEY cells by reducing the expression of RhoA and Rac, and it blockaded invation by down-regulating the protein level of MMP-9 and -2, and up-regulating the protein level of TIMP-1 and -2, thus reduced the activities of MMP-9 and -2 confirmed by gelatin zymography assay. In summary, our finding demonstrated that garcinone E exerts anticancer activities by inducing apoptosis, and suppressing migration and invasion on ovarian cancer cells, which demonstrated certain therapeutic potential of garcinone E and relative mangosteen xanthone derivatives on ovarian cancer.
Keyword	Ovarian cancer garcinone E apoptosis metastasis ER stress
Language	英語English
The Source to Article	PB_Publication
PUB ID	34448
Document Type	Conference paper
Collection	Institute of Chinese Medical Sciences
Corresponding Author	Lu, J.
Recommended Citation GB/T 7714	Xu, XH,Li, T,Zhang, LL,et al. Garcinone E exerts anticancer properties on ovarian cancer cells[C], 2016.
APA	Xu, XH., Li, T., Zhang, LL., Chen, X., Guo, X., Chen, X. P.., Wang, Y. T.., Lin, L.., & Lu, J. (2016). Garcinone E exerts anticancer properties on ovarian cancer cells. 15th Meeting of Consortium for Globalization of Chinese Medicine.

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