The "Mysterious" transcription and translation activities inside Aspergillus dormant spores.

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	The "Mysterious" transcription and translation activities inside Aspergillus dormant spores.
	Wang, F.; Sethiya, P.; Wong, K. H.
	2018-06-08
Source Publication	Macau Symposium on Biomedical Sciences
Abstract	Conidia spores are specialized asexual reproductive cells of filamentous fungi capable of staying dormant for long time. Previous studies showed that many mRNAs are present inside fungal spores, but it is unknown whether the mRNAs are prepackaged in spores during conidiation or transcription is actively ongoing inside the so-called dormant spores. We found that hundreds of genes have high RNA polymerase II bindings in spores even after 3-6 days post-conidiation and that different genes are bound when spores are exposed to different conditions (e.g. Nacl+, Zn-, 37℃, 4℃, 42℃), suggesting that spores are transcriptionally active and responsive. Surprisingly, genome-wide TBP profiling (an assay for mRNAs and tRNAs transcription) suggests that global tRNAs synthesis and presumably translation are absent in spores. The high mRNAs synthesis without translation raises an interesting question about the physiological roles of spores’ transcription. We speculate that the transcription is to synthesize and pre-load necessary mRNAs in spores according to their encounters in the environment and that the spores “experience” (i.e. the accumulated mRNAs) endow them different germination properties and fitness under different environments. We are on the way of testing how spores’ “experience” affect their environmental response. On the other hand, the lack of tRNAs synthesis in spore prompt us to investigate when tRNAs initiate synthesise during spores’ germination. Strikingly, we found a drastic increase (>10 fold) of TBP binding at tRNAs within minutes after spores are induced to germinate. This result not only suggests a novel sensitive way of measuring early germination response to test our “experience” hypothesis, but also highlights yet another important but unexplored aspect of spore biology that is the regulation of tRNAs biosynthesis during germination. The overall findings will have significant impacts for new antifungal targets research.
Keyword	transcription translation Aspergillus spores
Language	英語English
The Source to Article	PB_Publication
PUB ID	40076
Document Type	Conference paper
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES Faculty of Health Sciences
Corresponding Author	Wong, K. H.
Recommended Citation GB/T 7714	Wang, F.,Sethiya, P.,Wong, K. H.. The "Mysterious" transcription and translation activities inside Aspergillus dormant spores.[C], 2018.
APA	Wang, F.., Sethiya, P.., & Wong, K. H. (2018). The "Mysterious" transcription and translation activities inside Aspergillus dormant spores.. Macau Symposium on Biomedical Sciences.

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