The 4th MSBS

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Status	已發表Published
	The 4th MSBS
	Sun, H.; Miao, Z.; Zhang, X.; Chan, U.I.; Su, S.S.M; Guo, S.; Wong, K. H.; Xiaoling, X.; Chuxia, D.
	2017-06-21
Source Publication	4th Macau Symposium on Biomedical Sciences 2017
Abstract	The development of female mammary glands usually starts during puberty and is dramatically enhanced in pregnancy, as the result of accelerated proliferation and differentiation of mammary stem cells (MaSCs) in response to sexual hormones stimulation. Although the hierarchy of mammary epithelia has been studied by marker analysis and cell fate tracing, the molecular characters of MaSCs remain elusive, partly due to lack of exclusive markers for MaSCs and undiscovered transcriptome complexity via conventionally ensemble measurements of gene expression in bulk of cells. Hence, in this study we performed comprehensive and high-resolution transcriptome analysis of 239 single mammary basal and luminal epithelial cells from virgin and pregnant mice. Based on their respective gene expression profiles, we unbiasedly classified the single mammary cells into seven distinct subgroups with typical markers and enriched biological functions, indicating their locations and properties in mammary hierarchy. Moreover, pseudo-temporally reconstructed mammary epithelia developmental trajectory reveals unidirectional and continuous differentiation of MaSCs in virgin state but bidirectional differentiation into basal and luminal lineages separately in pregnant mice. In sum, this study provides significant insights into heterogeneity and hierarchy of mouse mammary epithelia.
Keyword	mammary stem cells Single cell RNAseq
Language	英語English
The Source to Article	PB_Publication
PUB ID	32311
Document Type	Conference paper
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES Faculty of Health Sciences
Corresponding Author	Chuxia, D.
Recommended Citation GB/T 7714	Sun, H.,Miao, Z.,Zhang, X.,et al. The 4th MSBS[C], 2017.
APA	Sun, H.., Miao, Z.., Zhang, X.., Chan, U.I.., Su, S.S.M., Guo, S.., Wong, K. H.., Xiaoling, X.., & Chuxia, D. (2017). The 4th MSBS. 4th Macau Symposium on Biomedical Sciences 2017.

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