Effects of garcinone E on ovarian cancer cells: Endoplasmic reticulum stress, apoptosis, migration and invasion inhibition

UM > Institute of Chinese Medical Sciences

Status	已發表Published
	Effects of garcinone E on ovarian cancer cells: Endoplasmic reticulum stress, apoptosis, migration and invasion inhibition
	Xu, XH; Li, T; Zhang, LL; Chen, X; Guo, X; Chen, X. P.; Wang, Y. T.; Lin, L.; Lu, J.
	2016-10-01
Source Publication	The 14th National Academic Conference of Chinese Pharmacological Society
Abstract	OBJECTIVE: Garcinone E, a xanthone derivative isolated from mangosteen, has been reported to have cytotoxic capacities. We aim to study the anticancer effects of garcinone E on ovarian cancer cells. METHODS: MTT assay, LDH release assay, Hoechst 33342 staining, Annexin V/PI staining, and JC-1 staining were used to test the antitumor abilities on several ovarian cancer cell lines, and wound-healing assay, transwell assay, and gelatin zymography were carried out to examine the migratory and invasive effects of malignant ovarian cancer HEY cells. Relevant mRNA transcription and protein expression were evaluated by western blotting. RESULTS: Garcinone E treatment significantly inhibited the proliferation and caused cell death on ovarian cancer HEY, A2780 as well as A2780-palicitaxel resistant cells. It induced endoplasmic reticulum stress in HEY cells and activated IRE signaling pathway, which provided protection on HEY cells. Meanwhile, knocking down of IRE-1α by siRNA further activated caspase cascade and caused more cell death. On the other hand, garcinone E eliminated migrative ability of HEY cells by reducing the expression of RhoA and Rac, and it blockaded invasion by down-regulating the protein level of MMP-9 and -2, and up-regulating the protein level of TIMP-1 and -2. Garcinone E reduced activities of MMP-9 and -2 were confirmed by gelatin zymography assay. CONCLUSION: Our finding demonstrated that garcinone E exerts anticancer activities by inducing apoptosis, and suppressing migration and invasion on ovarian cancer cells, which demonstrated certain therapeutic potential of garcinone E on ovarian cancer.
Keyword	Ovarian cancer garcinone E apoptosis metastasis ER stress
Language	英語English
The Source to Article	PB_Publication
PUB ID	36493
Document Type	Conference paper
Collection	Institute of Chinese Medical Sciences
Corresponding Author	Lu, J.
Recommended Citation GB/T 7714	Xu, XH,Li, T,Zhang, LL,et al. Effects of garcinone E on ovarian cancer cells: Endoplasmic reticulum stress, apoptosis, migration and invasion inhibition[C], 2016.
APA	Xu, XH., Li, T., Zhang, LL., Chen, X., Guo, X., Chen, X. P.., Wang, Y. T.., Lin, L.., & Lu, J. (2016). Effects of garcinone E on ovarian cancer cells: Endoplasmic reticulum stress, apoptosis, migration and invasion inhibition. The 14th National Academic Conference of Chinese Pharmacological Society.

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