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Clathrin and AP-1 mediate apical polarity and lumen formation in C. elegans tubulogenesis
Zhang, H.; Kim, A.; Abraham, N.; Hall, D.; Fleming, J.; Gobel, V.
2012-04-25
Source PublicationDevelopment
ISSN0951-1991
Pages2071-2083
AbstractClathrin coats vesicles in all eukaryotic cells and has a well-defined role in endocytosis, moving molecules away from the plasma membrane. Its function on routes towards the plasma membrane was only recently appreciated and is thought to be limited to basolateral transport. Here, an unbiased RNAi-based tubulogenesis screen identifies a role of clathrin (CHC-1) and its AP-1 adaptor in apical polarity during de novo lumenal membrane biogenesis in the C. elegans intestine. We show that CHC-1/AP-1-mediated polarized transport intersects with a sphingolipid-dependent apical sorting process. Depleting each presumed trafficking component mislocalizes the same set of apical membrane molecules basolaterally, including the polarity regulator PAR-6, and generates ectopic lateral lumens. GFP::CHC-1 and BODIPY-ceramide vesicles associate perinuclearly and assemble asymmetrically at polarized plasma membrane domains in a co-dependent and AP-1-dependent manner. Based on these findings, we propose a trafficking pathway for apical membrane polarity and lumen morphogenesis that implies: (1) a clathrin/AP-1 function on an apically directed transport route; and (2) the convergence of this route with a sphingolipid-dependent apical trafficking path.
KeywordCaenorhabditis elegans Polarity Tubulogenesis Clathrin AP-1 Sphingolipids
URLView the original
Language英語English
The Source to ArticlePB_Publication
PUB ID53814
Document TypeJournal article
CollectionDEPARTMENT OF BIOMEDICAL SCIENCES
Corresponding AuthorGobel, V.
Recommended Citation
GB/T 7714
Zhang, H.,Kim, A.,Abraham, N.,et al. Clathrin and AP-1 mediate apical polarity and lumen formation in C. elegans tubulogenesis[J]. Development, 2012, 2071-2083.
APA Zhang, H.., Kim, A.., Abraham, N.., Hall, D.., Fleming, J.., & Gobel, V. (2012). Clathrin and AP-1 mediate apical polarity and lumen formation in C. elegans tubulogenesis. Development, 2071-2083.
MLA Zhang, H.,et al."Clathrin and AP-1 mediate apical polarity and lumen formation in C. elegans tubulogenesis".Development (2012):2071-2083.
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