ENDOGENOUS IGF SIGNALING DIRECTS HETEROGENEOUS MESOENDODERM DIFFERENTIATION IN HUMAN EMBRYONIC STEM CELLS

UM > Faculty of Health Sciences > DEPARTMENT OF BIOMEDICAL SCIENCES

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	ENDOGENOUS IGF SIGNALING DIRECTS HETEROGENEOUS MESOENDODERM DIFFERENTIATION IN HUMAN EMBRYONIC STEM CELLS
	Chen, G.; Yang, Y.; Ren, Z.; Liu, W.
	2019-06-26
Source Publication	ISSCR 2019 Annual Meeting
Abstract	In a successful embryogenesis process, various cell types often emerge simultaneously from their common progenitors under the influence of intrinsic signals. In human embryonic stem cell (hESC) differentiation, mesoendoderm progenitor cells spontaneously generate multiple cell types, making them an excellent system to understand the molecular regulations that maintain the balance of different cell types in embryogenesis. In this report, we demonstrate that IGF proteins are endogenously expressed during differentiation, and contribute to the cell fate diversity from mesoendoderm progenitors. Small chemical LY294002 inhibits the IGF pathway to suppress epicardial and noncardiac cell fates, and promotes cardiomyocyte differentiation. LY294002 leads to distinct gene expression profile in cardiomyocytes, and provides a valuable addition to conventional differentiation methods. Further study shows that LY294002 inhibits CK2 pathway to induce cardiomyocyte cell fate, and CK2 inhibitor such as apigenin also efficiently promotes cardiomyocyte differentiation. This study elucidates the crucial roles of endogenous IGF in mesoderm differentiation, and the inhibition of IGF pathway is an effective approach to generate cardiomyocyte for hESC applications.
Keyword	Insulin IGF hESC CK2
Language	英語English
The Source to Article	PB_Publication
PUB ID	47177
Document Type	Conference paper
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES
Corresponding Author	Chen, G.
Recommended Citation GB/T 7714	Chen, G.,Yang, Y.,Ren, Z.,et al. ENDOGENOUS IGF SIGNALING DIRECTS HETEROGENEOUS MESOENDODERM DIFFERENTIATION IN HUMAN EMBRYONIC STEM CELLS[C], 2019.
APA	Chen, G.., Yang, Y.., Ren, Z.., & Liu, W. (2019). ENDOGENOUS IGF SIGNALING DIRECTS HETEROGENEOUS MESOENDODERM DIFFERENTIATION IN HUMAN EMBRYONIC STEM CELLS. ISSCR 2019 Annual Meeting.

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