Residential College | false |
Status | 已發表Published |
Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11 | |
Man Cheng1; Ceci W.L.Chan1; Randy C.F.Cheung1; Rama Kamesh Bikkavilli1; Qi Zhao1; Shannon W.N.Au1; Paul K.S.Chan2; Susanna S.T.Lee1; Gregory Cheng3; Walter K.K.Ho1; Wing-Tai Cheung1 | |
2005 | |
Source Publication | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS |
ISSN | 0006-291X |
Volume | 338Issue:3Pages:1654-1660 |
Abstract | Infection of SARS-associated coronavirus (SARS-CoV) induced a strong anti-nucleocapsid (anti-N) antibody response. However, the pathophysiological significance of the anti-N antibodies in SARS pathogenesis is largely unknown. To profile the anti-N antibodies, a phage-displayed scFv library was prepared from mice immunized with heat-inactivated SARS-CoV-infected Vero E6 cell lysate. Specific anti-N scFvs were isolated by panning against a recombinant nucleocapsid protein and reactivity was confirmed with phage-ELISA. Sequence analysis indicated that two of the isolated anti-N scFv clones were identical and displayed a high homology with an scFv specific for interleukin 11 (IL-11), an anti-inflammatory cytokine derived from bone marrow stroma cells. In a neutralization assay, IL-11-induced STAT 3 phosphorylation in rat intestinal epithelial IEC-18 cells was completely suppressed by the anti-N scFv clone L9N01. |
Keyword | Antibody Interleukin 11 Nucleocapsid Protein Phage-display Sars-coronavirus Scfv |
DOI | 10.1016/j.bbrc.2005.10.088 |
Indexed By | SCIE |
Language | 英語English |
WOS Research Area | Biochemistry & Molecular Biology ; Biophysics |
WOS Subject | Biochemistry & Molecular Biology ; Biophysics |
WOS ID | WOS:000233710100047 |
Scopus ID | 2-s2.0-27744574606 |
Fulltext Access | |
Citation statistics | |
Document Type | Journal article |
Collection | Faculty of Health Sciences |
Corresponding Author | Wing-Tai Cheung |
Affiliation | 1.Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China 2.Department of Microbiology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China 3.Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China |
Recommended Citation GB/T 7714 | Man Cheng,Ceci W.L.Chan,Randy C.F.Cheung,et al. Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 338(3), 1654-1660. |
APA | Man Cheng., Ceci W.L.Chan., Randy C.F.Cheung., Rama Kamesh Bikkavilli., Qi Zhao., Shannon W.N.Au., Paul K.S.Chan., Susanna S.T.Lee., Gregory Cheng., Walter K.K.Ho., & Wing-Tai Cheung (2005). Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 338(3), 1654-1660. |
MLA | Man Cheng,et al."Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 338.3(2005):1654-1660. |
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