Introduction A growing body of evidence demonstrates that minimal volume of high-intensity interval training and resistance training can serve as effective alternates to traditional time-consuming training, inducing similar or even superior physiological adaptations (Babraj et al., 2009). However, limited information is available regarding the impact of minimal volume exercise on glucose metabolism. The study aimed to analyze glucose regulation responses in obese adults following minimal volume high-intensity interval exercise (HIE) and resistance exercise (RES). Methods Fifteen obese adults (age: 21.3±2.4 years; BMI: 34.1±4.8) underwent: (1) HIE: all-out cycling exercise 30 sec × 4, interspersed with 4 min of rest, (total 14 min); (2) RES: a circuit of nine resistance exercise involved the large muscle groups with ten repetitions, interval with 1 min of rest (total 14 min); and (3) CON: a control session of no exercise, (total 14 min). They completed an oral glucose tolerance test (OGTT) ~12 hours after exercises or CON. Blood was collected before and every 30 minutes during the OGTT and was analyzed for glucose and insulin. The HIE, RES and CON sessions were carried out in random order at the same time of day and separated by at least 7 days. Results Blood glucose (mol.l-1) at 30 min during the OGTT in HIE (8.63 ± 1.41) trial was significantly lower (p<0.05) than the corresponding values of CON (9.26 ± 0.37), but not in blood insulin (175.4 ± 71.2 vs. 173.6±79.4 nU.l-1, p>0.05) at the same time point. Further, there were no differences in glucose or insulin between conditions for other single time points or as area under the curve. Discussion The results of the present study demonstrate that neither exercise produced substantial enhanced glucose removal compared to control, despite the lower level in glucose at a single time point in HIE. It was noteworthy that the marked heterogeneity in glucose and insulin responses during OGTT, even our sample included several non-responders. This study extends the previous literature showing the metabolic beneficial effects of classic time-consuming exercise (Wojtaszewski et al., 2003) by showing that the substantial inter-individual variability in glucose regulation responses to time-efficient exercise intervention strategies in this population might be at risk for the development of metabolic diseases.