| Status | 已發表Published |
| Comprehensive analysis of chromatin landscape in filamentous Aspergillus nidulans. | |
Ho, J.W.K; Djordjevic, D.D; Wang, X.; Miao, Z.; Parsania, C.; Tan, K. ; Wong, K. H.
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| 2015-07-12 | |
| Source Publication | Comprehensive Analysis Of Chromatin Landscape In Filamentous Aspergillus Nidulans.
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| Publication Place | Dublin, Ireland |
| Abstract | Chromatin organisation, such as the deposition of active or repressive histone modifications, plays an important role in regulating gene expression. Advances in ChIP-seq and associated bioinformatics techniques have enabled genome-wide analysis of chromatin modification and transcription regulation dynamics. The chromatin landscape of human, mouse, and several model organisms have been widely studied, but other medically and biotechnologically important species, including many fungal species, are not yet fully studied. Here we present a genome-wide chromatin landscape of an important filamentous fungal model organism, Aspergillus nidulans. Using ChIP-seq, we generated genome-wide profiles of H3, H3K4me3, H3K4me1, H3K27ac, H3K9me3, H3K9ac, PolII and transcription factors in A. nidulans under different nutrient availability and quality as a novel approach to dissect the regulation of cellular metabolic homeostasis. We used a recently developed hierarchically linked infinite hidden Markov model (hiHMM) to systematically discover and characterise the most prevalent combination of histone modifications, i.e., chromatin state. Our analysis revealed an interesting new chromatin state that consists of both H3K9me3 and H3K27ac, and is associated with gene repression. This state is covering 15-20% of the genome, and is located most prevalently in sub-telomeric regions, covering secondary metabolism and transmembrane transport genes. In addition, 10-20% of the genome are marked by classical enhancer chromatin marks, suggesting that these previously uncharacterised regions may have potential regulatory functions. Our work represents a valuable resource in A. nidulans that opens new avenues for investigation of the dynamic chromatin organisation and gene regulation in fungi. |
| Keyword | intelligent systems |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 14939 |
| Document Type | Conference paper |
| Collection | DEPARTMENT OF BIOMEDICAL SCIENCES |
| Corresponding Author | Wong, K. H. |
| Recommended Citation GB/T 7714 | Ho, J.W.K,Djordjevic, D.D,Wang, X.,et al. Comprehensive analysis of chromatin landscape in filamentous Aspergillus nidulans.[C], Dublin, Ireland, 2015. |
| APA | Ho, J.W.K., Djordjevic, D.D., Wang, X.., Miao, Z.., Parsania, C.., Tan, K.., & Wong, K. H. (2015). Comprehensive analysis of chromatin landscape in filamentous Aspergillus nidulans.. Comprehensive Analysis Of Chromatin Landscape In Filamentous Aspergillus Nidulans.. |
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