Solasodine induces apoptosis and inhibits metastasis on ovarian cancer HEY cells

UM > Institute of Chinese Medical Sciences

Status	已發表Published
	Solasodine induces apoptosis and inhibits metastasis on ovarian cancer HEY cells
	Xu, XH; Zhang, LL; Wu, GS; Chen, X; Li, T; Liu, JL; Chen, X. P.; Wang, Y. T.; Lu, J.
	2015-11-01
Source Publication	The 13th National Academic Conference of Chinese Pharmacological Society
Abstract	Aim Solasodine, a steroidal alkaloid isolated from solanaceous species, has been reported to exhibit anticancer activity on several cell lines. The aim of this study is to explore the antitumor potential of solasodine on ovarian cancer HEY cells. Methods MTT assay, Hoechst 33342 staining, JC-1 mitochondrial membrane potential assay, and LDH release assay were used to study the anti-proliferative and apoptosis-inducing effects of solasodine. MDC staining was to label the acidic puncta in the cells. Meanwhile, wound healing assay and transwell assay were performed to determine whether solasodine exhibits anti-metastasis effect on HEY cells. Gelatin zymography assay was used to determine the activities of MMP-2 and MMP-9 and western blotting was used to examine the expression of the relevant proteins. Results Solasodine inhibited the cell viability on HEY cells in time- and dose-dependent manners, causing shrinking of nuclei, damage of cell membrane, and appealing apoptotic body on high dosage and reducing cell mitochondrial membrane potential. It also inhibited autophagic flux that interfere autolysosome degradation. Furthermore, solasodine suppressed the migration and invasion abilities of HEY cells ascribable to down-regulating the expression and attenuating the activities of MMP-2 and MMP-9. Conclusion The inducing apoptosis and anti-metastasis activity of solasodine on HEY cells suggest it appears certain potential on ovarian cancer treatment.
Keyword	ovarian cancer solasodine apoptosis autophagy migration invasion
Language	英語English
The Source to Article	PB_Publication
PUB ID	34461
Document Type	Conference paper
Collection	Institute of Chinese Medical Sciences
Corresponding Author	Lu, J.
Recommended Citation GB/T 7714	Xu, XH,Zhang, LL,Wu, GS,et al. Solasodine induces apoptosis and inhibits metastasis on ovarian cancer HEY cells[C], 2015.
APA	Xu, XH., Zhang, LL., Wu, GS., Chen, X., Li, T., Liu, JL., Chen, X. P.., Wang, Y. T.., & Lu, J. (2015). Solasodine induces apoptosis and inhibits metastasis on ovarian cancer HEY cells. The 13th National Academic Conference of Chinese Pharmacological Society.

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