Alisol B induces endoplasmic reticulum stress and activates a protective NF-κB nuclear translocation in colorectal cancer cells

UM > Institute of Chinese Medical Sciences

Status	已發表Published
	Alisol B induces endoplasmic reticulum stress and activates a protective NF-κB nuclear translocation in colorectal cancer cells
	Liu, YF; Chen, X. P.; Wang, Y. T.; Lu, J.
	2016-11-01
Source Publication	The Fourth Symposium on New Trends in Individualized Therapy and Antineoplastic Agents
Abstract	Objective: Emerging evidence suggests that the natural products have the bioactivity to induce cancer cells apoptotic death and have the potential therapeutic effects on colorectal cancer. In this study, we identified alisol B as an endoplasmic reticulum (ER) stress inducer and found it could activate the NF-κB nuclear translocation to protect cells from apoptotic death. Methods: MTT assay and LDH assay were used to test the cytotoxicity of alisol B on colorectal cancer HCT116 cells. The morphological changes were shown, and the Annexin V/PI staining and western blot assay were used to assess the apoptotic effects of alisol B. Western blot was used to determine the expression of proteins. Results: Alisol B inhibited cell proliferative inhibition and induced apoptosis in HCT116 cells. The endoplasmic reticulum (ER) stress was induced after cells being treated with alisol B for 24 h, especially the increased protein levels of CHOP, which is a hallmaker of ER stress. The activated NF-κB nuclear translocation, which exerted the protective effect, was observed when HCT116 cells were exposed to alisol B. Conclusion: Our work provides novel insights into the molecular mechanism of alisol B-induced apoptosis in colorectal cancer cells.
Keyword	Alisol B ER stress NF-κB CHOP
Language	英語English
The Source to Article	PB_Publication
PUB ID	36498
Document Type	Conference paper
Collection	Institute of Chinese Medical Sciences
Corresponding Author	Lu, J.
Recommended Citation GB/T 7714	Liu, YF,Chen, X. P.,Wang, Y. T.,et al. Alisol B induces endoplasmic reticulum stress and activates a protective NF-κB nuclear translocation in colorectal cancer cells[C], 2016.
APA	Liu, YF., Chen, X. P.., Wang, Y. T.., & Lu, J. (2016). Alisol B induces endoplasmic reticulum stress and activates a protective NF-κB nuclear translocation in colorectal cancer cells. The Fourth Symposium on New Trends in Individualized Therapy and Antineoplastic Agents.

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