Platycodin D induces apoptosis and triggers autophagy in hepatocellular carcinoma Bel-7402 cells

UM > Institute of Chinese Medical Sciences

Status	已發表Published
	Platycodin D induces apoptosis and triggers autophagy in hepatocellular carcinoma Bel-7402 cells
	Li, T; Xu, XH; Tang, ZH; Wang, Y. T.; Chen, Y; Lu, J.
	2015-04-01
Source Publication	Chinese Society for Cell Biology-2015 National Congress and Academic Conference
Abstract	Platycodin D (PD) is the main saponin isolated from the traditional Chinese herb Platycodon radix. It exhibits anticancer activity in several types of cancer cell lines. Present study revealed that PD not only inhibited the cell proliferation in vitro but also retarded the growth of xenografts of hepatocellular carcinoma Bel-7402 cells. The increased apoptotic cells induced by PD were observed by using Annexin V/PI staining assay and Hoechst 33342 staining assay, respectively. The protein levels of cleaved PARP and cleaved caspase-3 were upregulated, while that of Bcl-2 was decreased after PD treatment. Moreover, PD triggered autophagy as evidenced by the formation of autophagic vacuoles, upregulation of microtubule-associated protein 1 light chain 3 II, and the elevation of MDC-positive cells. Besides, PD-induced cell death was enhanced when combined with autophagy inhibitor, chloroquine or bafilomycin A1. These findings suggest that the combination with autophagy inhibitor and PD might be a promising strategy for the treatment of hepatocellular carcinoma cells.
Keyword	platycodin D Bel-7402 apoptosis autophagy in vivo
Language	英語English
The Source to Article	PB_Publication
PUB ID	34458
Document Type	Conference paper
Collection	Institute of Chinese Medical Sciences
Corresponding Author	Lu, J.
Recommended Citation GB/T 7714	Li, T,Xu, XH,Tang, ZH,et al. Platycodin D induces apoptosis and triggers autophagy in hepatocellular carcinoma Bel-7402 cells[C], 2015.
APA	Li, T., Xu, XH., Tang, ZH., Wang, Y. T.., Chen, Y., & Lu, J. (2015). Platycodin D induces apoptosis and triggers autophagy in hepatocellular carcinoma Bel-7402 cells. Chinese Society for Cell Biology-2015 National Congress and Academic Conference.

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