Deficiency in Fpr2 results in reduced number of Lin-c-Kit+Sca1+ myeloid progenitor cells

UM > Institute of Chinese Medical Sciences

Status	已發表Published
	Deficiency in Fpr2 results in reduced number of Lin-c-Kit+Sca1+ myeloid progenitor cells
	Chen, K; Singh, V; Tang, P; Bao, Z; He, T.; Xiang, Y; Gong, W; Toshimura, T.; Le, Y; Tessarollo, L; Chen, X.; Wang, J
	2018-08-31
Source Publication	J Biol Chem
ISSN	0021-9258
Pages	13452-13463
Abstract	Lin-c-Kit+ Sca-1+ cell population in the bone marrow (BM) serves as the direct precursors for differentiation of myeloid cells. In this study, we report that deficiency in Fpr2, a G-protein coupled chemoattractant receptor in mice, is associated with reduced BM nucleated cells including CD31+Ly6C+ (granulocytes and monocytes), CD31-/Ly6Cint (granuloid cells), and CD31-/Ly6Chigh (predominantly monocytes) cells. In particular, the number of Lin-c-Kit+Sca-1+ (LKS) cells was reduced in Fpr2-/- mouse BM. This was supported by observations of the reduced incorporation of intraperitoneally injected BrdU by cells in the c-Kit+ population from Fpr2-/- mouse BM. Purified c-Kit+ cells from Fpr2-/- mice showed reduced expansion when cultured in vitro with stem cell factor (SCF). SCF/c-Kit-mediated phosphorylation of P38, STAT1, Akt (Thr308) and Akt (Ser473) was also significantly reduced in c-Kit+ cells from Fpr2-/- mice. Furthermore, Fpr2 agonists enhanced SCF-induced proliferation of c-Kit+ cells. Colony-Forming Unit Assay revealed that CFU-GM formation of BM cells from Fpr2-/- mice was significantly reduced. After heat-inactivated bacterial stimulation in the airway, the expansion of c-kit+ Sca-1+ cells in BM and recruitment of Ly6G+ cells to the lungs and CD11b+Ly6C+TNF-α+ cells to the spleen of Fpr2-/- mice were significantly reduced. These results demonstrate an important role for Fpr2 in the development of myeloid lineage precursors in mouse BM.
Keyword	Mouse Bone marrow Lin-c-Kit+Sca-1+ Proliferation Fpr2 SCF c-Kit
URL	View the original
Language	英語English
The Source to Article	PB_Publication
PUB ID	40434
Document Type	Journal article
Collection	Institute of Chinese Medical Sciences
Recommended Citation GB/T 7714	Chen, K,Singh, V,Tang, P,et al. Deficiency in Fpr2 results in reduced number of Lin-c-Kit+Sca1+ myeloid progenitor cells[J]. J Biol Chem, 2018, 13452-13463.
APA	Chen, K., Singh, V., Tang, P., Bao, Z., He, T.., Xiang, Y., Gong, W., Toshimura, T.., Le, Y., Tessarollo, L., Chen, X.., & Wang, J (2018). Deficiency in Fpr2 results in reduced number of Lin-c-Kit+Sca1+ myeloid progenitor cells. J Biol Chem, 13452-13463.
MLA	Chen, K,et al."Deficiency in Fpr2 results in reduced number of Lin-c-Kit+Sca1+ myeloid progenitor cells".J Biol Chem (2018):13452-13463.

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