| Status | 已發表Published |
| The acetylation of histone 3 is involved in the neuroprotective effects of artemisinin in PC12 cells | |
Chen, Y. ; Zheng, W. 
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| 2021-07-01 | |
| Source Publication | 7th macau symposium on Biomedical Science
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| Abstract | Alzheimer's disease (AD) is a progressive degenerative disorder of the nervous system. It is characterized by a comprehensive dementia phenotype consisting of memory impairment, aphasia, apraxia, agnosia, impairment of visuospatial skills, executive dysfunction, and personality and behavioral changes. At present, there is no specific medicine that can cure or effectively reverse this disease, it is urgently to find drugs to treat or relieve this disease. In our lab, we have reported that artemisinin, an anti-malarial drug discovered by Prof. Tu Youyou, shown promising neuroprotective effects on neuronal cells and is capable of preventing Alzheimer's disease. Moreover, its mechanism of action involves the phosphorylation of AMPK and ERK1/2. However, the mechanism of action of artemisinin is not single. Here we reported that artemisinin may protect against Alzheimer's disease by regulating the acetylation levels of histones. First, Aβ1-42 induced cell injury was used to mimics Alzheimer's disease in vitro. Then, artemisinin pretreatment followed by addition of Aβ1-42 in PC12 cells determined the pre - protective effect of artemisinin on PC12 cells. Moreover, we found that artemisinin pretreatment gradually increased the acetylated histone 3 expression level in a time and dose dependent manner. Meanwhile, pretreated with p300i, an inhibitor of acetylase, decreased the expression of acetylation of histone 3, thereby decreasing cell viability and increasing cell apoptosis. When pretreated with TSA, an inhibitor of deacetylase, the activity of deacetylases is attenuated, allowing histone 3 deacetylation levels to be suppressed and thereby elevating the expression of acetylation. The PC12 cells showed enhanced cell viability and decreased cell apoptosis. Therefore, we reported that artemisinin could protect PC12 cells from Aβ1-42 induced cell injury by increasing the acetylation level of histone 3. At least, acetylation of histones 3 is one such pathway by which artemisinin prevents Alzheimer's disease. Through the above experiments, we discovered a novel mechanism for artemisinin to protect neuronal cells, which provide a new avenue for targeted therapy of Alzheimer's disease and provides the possibility for the development of new drugs. |
| Keyword | Artemisinin Alzheimer's disease acetylation histone 3 |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 57983 |
| Document Type | Conference paper |
| Collection | DEPARTMENT OF PHARMACEUTICAL SCIENCES |
| Corresponding Author | Zheng, W. |
| Recommended Citation GB/T 7714 | Chen, Y.,Zheng, W.. The acetylation of histone 3 is involved in the neuroprotective effects of artemisinin in PC12 cells[C], 2021. |
| APA | Chen, Y.., & Zheng, W. (2021). The acetylation of histone 3 is involved in the neuroprotective effects of artemisinin in PC12 cells. 7th macau symposium on Biomedical Science. |
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