Objective： Inflammation during cerebral IR injury has been associated with increased IL-1β synthesis, which reduces cerebral blood flow, promotes free radical release, and enhances toxic effect of excitatory amino acids. In this study the effects of bioactive components of Danhong Injection (DHI) on inflammatory factors IL-1β were investigated. Methods： Sprague-Dawley (SD) rats were randomly divided into 4 equal groups (each n = 6) (sham operations (control), cerebral IR damage alone (model group), IR model treated with DHI (treatment group), and IR model treated with nimodipine (positive control). The drugs concentration of Danshensu (DSS) and Hydroxysafflor yellow A (HSA) in Plasma were measured by HPLC at 5, 15, 30, 60, 120, 180, 240, and 360 min. The levels of IL-1β in Plasma were measured by enzyme-linked immunosorbent assay. Curves were fit using non-linear least squares methods. Results The results show that levels of IL-1β were decreased after 60, 120, 180, 240, and 360 min (P < 0.05) and infarct volume (%) was reduced compared to the model group (P < 0.01). Regression equation of DSS and HSA showed that IL-1β and DSS were better fit than those for HSA. DHI reduces inflammation in cerebral ischemic injury. And the DSS in DHI mainly reduce IL-1β in a rat model of cerebral ischemia/reperfusion.