| Status | 已發表Published |
| H2O2 promotes the growth of retinal neuron through JNK/p38 MAPK pathway | |
Zheng, W. 
| |
| 2015-03-22 | |
| Source Publication | BIT’S6THAnnual World Congress of Neurotalk
 |
| Abstract | Purpose: To study effect of hydrogen peroxide(H2O2) on the apoptosis of retinal GRC-5 cellls induced by serum deprivation and the underlying mechanism. Method:GRC-5 cells deprived of serum were used as a toxicity cellular model. The effect of H2O2 on the viability and apoptosis of GRC-5 cells was evaluated by MTT and Hoechst staining assays. The activation of kinases was determined by Western blotting with specific phospho-antibodies. Kinases or proteins involved were investigated by the application of kinase specific inhibitors or specific siRNA. Result: Serum deprivation induced apoptosis of GRC-5 cells. Low concentrations of H2O2 (0-30um) blocked serum deprivation induced apoptosis of GRC-5 cells while a toxic effect was observed at higher concentration of H202 (>60um). Western blotting showed that 30um H2O2significantly stimulated the activation of JNK and p38 MAPK while it had no effect on the activation of Akt, ERK1/2 and other survival kinase. Application of JNK or p38 MAPK specific inhibitors inhibited the protective effect of H2O2 although JNK inhibitors were more potent than inhibitors of P38 MAPK. Moreover, 30um H202 induced the phosphorylation of c-jun time dependently. Blockade of the phosphorylation of c-jun induced by H202 on GRC-5 cells. Conclusion: The JNK/C-Jun and p38 MAPK pathways play important roles in the protective effect of low concentrations of H2O2 on serum deprivation induced injury of GRC-5 |
| Keyword | H2O2 apoptosis JNK p38 MAPK |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 19188 |
| Document Type | Conference paper |
| Collection | DEPARTMENT OF PHARMACEUTICAL SCIENCES |
| Corresponding Author | Zheng, W. |
| Recommended Citation GB/T 7714 | Zheng, W.. H2O2 promotes the growth of retinal neuron through JNK/p38 MAPK pathway[C], 2015. |
| APA | Zheng, W..(2015). H2O2 promotes the growth of retinal neuron through JNK/p38 MAPK pathway. BIT’S6THAnnual World Congress of Neurotalk. |
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