Three-Dimensional Segmentation and Quantitative Measurement of the Aqueous Outflow System of Intact Mouse Eyes Based on Spectral Twophoton Microscopy Techniques

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	Three-Dimensional Segmentation and Quantitative Measurement of the Aqueous Outflow System of Intact Mouse Eyes Based on Spectral Twophoton Microscopy Techniques
	Zhang, X.; Liu, N.; Mak, P. U.; Pun, S. H.; Vai, M. I.; Masihzadeh, O.; Kahook, M. Y.; Lei, T. C.; Ammar, D. A.
	2016-06-01
Source Publication	Invest Ophthalmol Vis Sci.
ISSN	1552-5783
Pages	3159-3167
Abstract	PURPOSE. To visualize and quantify the three-dimensional (3D) spatial relationships of the structures of the aqueous outflow system (AOS) within intact enucleated mouse eyes using spectral two-photon microscopy (TPM) techniques. METHODS. Spectral TPM, including two-photon autofluorescence (TPAF) and second-harmonic generation (SHG), were used to image the small structures of the AOS within the limbal region of freshly enucleated mouse eyes. Long infrared excitation wavelengths (930 nm) were used to reduce optical scattering and autofluorescent background. Image stacks were collected for 3D image rendering and structural segmentation. For anatomical reference, vascular perfusion with fluorescent-conjugated dextran (150 KDa) and trans-corneal perfusion with 0.1 lm fluorescent polystyrene beads were separately performed to identify the episcleral veins (EV) and the trabecular meshwork (TM) and Schlemm’s canal (SC), respectively. RESULTS. Three-dimensional rendering and segmentation of spectral two-photon images revealed detailed structures of the AOS, including SC, collector channels (CC), and aqueous veins (AV). The collagen of the TM was detected proximal to SC. The long and short axes ofthe SC were 82.2 6 22.2 lm and 6.7 6 1.4 lm. The diameters of the CC averaged 25.6 6 7.9 lm w
Keyword	second-harmonic generation two-photon fluorescence microscopy nonlinear microscopy glaucoma aqueous outflow
Language	英語English
The Source to Article	PB_Publication
PUB ID	19405
Document Type	Journal article
Collection	INSTITUTE OF MICROELECTRONICS
Recommended Citation GB/T 7714	Zhang, X.,Liu, N.,Mak, P. U.,et al. Three-Dimensional Segmentation and Quantitative Measurement of the Aqueous Outflow System of Intact Mouse Eyes Based on Spectral Twophoton Microscopy Techniques[J]. Invest Ophthalmol Vis Sci., 2016, 3159-3167.
APA	Zhang, X.., Liu, N.., Mak, P. U.., Pun, S. H.., Vai, M. I.., Masihzadeh, O.., Kahook, M. Y.., Lei, T. C.., & Ammar, D. A. (2016). Three-Dimensional Segmentation and Quantitative Measurement of the Aqueous Outflow System of Intact Mouse Eyes Based on Spectral Twophoton Microscopy Techniques. Invest Ophthalmol Vis Sci., 3159-3167.
MLA	Zhang, X.,et al."Three-Dimensional Segmentation and Quantitative Measurement of the Aqueous Outflow System of Intact Mouse Eyes Based on Spectral Twophoton Microscopy Techniques".Invest Ophthalmol Vis Sci. (2016):3159-3167.

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