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Patent NumberCN115754298A
Status申請中 Pending
膜载体斑点免疫渗滤测定噻虫嗪的试剂盒及其使用方法
Year Issued2023
2023-03-07
Application NumberCN202211498463.6
Application Date2022-11-28
Rights HolderUniversity of Macau
李鵬; 崔錫平; 魏金超; 盧健; 鐘章鋒; 王一濤
Country中國
Subtype发明专利 Invention
Abstract

本发明公开了一种膜载体斑点免疫渗滤测定噻虫嗪含量的试剂盒及其使用方法,该试剂盒中包括试纸条;试纸条包括固相载体以及包被于固相载体表面的噻虫嗪抗原;固相载体为硝酸纤维素膜或尼龙膜。本发明采用硝酸纤维素膜为固相载体,利用其印迹作用在其表面包被噻虫嗪包被抗原,在静电作用下将噻虫嗪特异性抗体与纳米金进行偶联标记,制备得到纳米金标记的噻虫嗪抗体,在膜载体定向渗滤作用下,噻虫嗪与噻虫嗪包被抗原竞争性结合纳米金标记的噻虫嗪抗体,通过判断膜上颜色的变化从而定性检测样本中噻虫嗪的含量。本发明较酶联免疫检测法的制备成本低廉,检测方法简便快速,检测成果明显直观,适合现场快速鉴定。

Document TypePatent
CollectionInstitute of Chinese Medical Sciences
Affiliation澳门大学
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
李鵬,崔錫平,魏金超,等. 膜载体斑点免疫渗滤测定噻虫嗪的试剂盒及其使用方法. CN115754298A[P]. 2023-03-07.
APA 李鵬., 崔錫平., 魏金超., 盧健., 鐘章鋒., & 王一濤 (2022-11-28). 膜载体斑点免疫渗滤测定噻虫嗪的试剂盒及其使用方法.
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