Simple Nano-Luciferase-Based Assay for the Rapid and High-Throughput Detection of SARS-CoV-2 3C-Like Protease

doi:10.1021/acs.analchem.2c02590

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	Simple Nano-Luciferase-Based Assay for the Rapid and High-Throughput Detection of SARS-CoV-2 3C-Like Protease
	Liu, Jingxin 1; Ge, Chenchen 1; Zha, Ling 1; Lin, Ligen2 ; Li, Rongsong 1
	2023-01-17
Source Publication	Analytical Chemistry
ISSN	0003-2700
Volume	95 Issue:2 Pages:714-719
Abstract	In this study, we described an easy-to-perform nano-luciferase (nLuc) sensor for the rapid detection of 3-chymotrypsin-like protease (3CLpro) encoded by SARS-CoV-2. The technology is based on the cleavage reaction of recombinant-nLuc via 3CLpro. The nLuc-based assay is a general, one-step method and is naturally specific in detection. The stability, sensitivity, detection range, and response time are fully characterized. The application of 3CLpro detection in artificial and human saliva as well as antiviral drug screening demonstrates that the method can quantify 3CLpro with high sensitivity in one step. With its unique features, the nLuc-based assay may find broad applications in the auxiliary diagnosis of SARS-CoV-2, as well as other types of coronavirus infection.
DOI	10.1021/acs.analchem.2c02590
URL	View the original
Indexed By	SCIE
Language	英語English
WOS Research Area	Chemistry
WOS Subject	Chemistry, Analytical
WOS ID	WOS:000954332800001
Publisher	AMER CHEMICAL SOC1155 16TH ST, NW, WASHINGTON, DC 20036
Scopus ID	2-s2.0-85145446179
Fulltext Access	View Full-Text via DOI View Full-Text via Web of Science View Full-Text via Scopus
Citation statistics
Document Type	Journal article
Collection	Faculty of Science and Technology Institute of Chinese Medical Sciences THE STATE KEY LABORATORY OF QUALITY RESEARCH IN CHINESE MEDICINE (UNIVERSITY OF MACAU)
Corresponding Author	Li, Rongsong
Affiliation	1.College of Health Science and Environmental Engineering, Shenzhen Technology University, Shenzhen, 3002 Lantian Road, Guangdong, 518118, China 2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, 999078, Macao
Recommended Citation GB/T 7714	Liu, Jingxin,Ge, Chenchen,Zha, Ling,et al. Simple Nano-Luciferase-Based Assay for the Rapid and High-Throughput Detection of SARS-CoV-2 3C-Like Protease[J]. Analytical Chemistry, 2023, 95(2), 714-719.
APA	Liu, Jingxin., Ge, Chenchen., Zha, Ling., Lin, Ligen., & Li, Rongsong (2023). Simple Nano-Luciferase-Based Assay for the Rapid and High-Throughput Detection of SARS-CoV-2 3C-Like Protease. Analytical Chemistry, 95(2), 714-719.
MLA	Liu, Jingxin,et al."Simple Nano-Luciferase-Based Assay for the Rapid and High-Throughput Detection of SARS-CoV-2 3C-Like Protease".Analytical Chemistry 95.2(2023):714-719.

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