Fungi can adapt and grow in diverse environments, the ability of which is mediated by complex sensing, signalling, and regulatory mecha- nisms. As a quick reversible covalent modification, protein phosphory- lation catalyzed by protein kinases plays essential roles in signal trans- duction and regulation of cellular processes. Despite their importance, the function of many kinases is still unknown even in well-studied model organisms such as A. nidulans, which has more than half of the non-essential kinases uncharacterized. In this work, we performed transcription profiling analysis on ninety-nine non-essential kinase mutants of A. nidulans grown in preferred carbon (e.g., glucose) and nitrogen (e.g., ammonium) conditions. The overall results reveal the transcriptional effects of each kinase and portray a function map of the A. nidulans kinome during active growth.