Humanization and directed evolution of the selenium-containing scFv phage abzyme

UM > Faculty of Health Sciences > DEPARTMENT OF BIOMEDICAL SCIENCES

Status	已發表Published
	Humanization and directed evolution of the selenium-containing scFv phage abzyme
	Xu, Y; Li, P; Nie, J; Zhao, Q.; Guan, S; Kuai, Z; Qiao, Y; Jiang, X; Li, Y; Li, W; Shi, Y; Kong, W; Shan, Y
	2018-05-01
Source Publication	RSC Advances
ISSN	2046-2069
Pages	17218-17223
Abstract	According to the binding site structure and the catalytic mechanism of the native glutathione peroxidase (GPX), three glutathione derivatives, GSH-S-DNP butyl ester (hapten Be), GSH-S-DNP hexyl ester (hapten He) and GSH-S-DNP hexamethylene ester (hapten Hme) were synthesized. By a four-round panning with a human synthetic scFv phage library against three haptens, the enrichment of the scFv phage particles with specific binding activity could be determined. Three phage particles were selected binding to each glutathione derivative, respectively. After a two-step chemical mutation to convert the serine residues of the scFv phage particles into selenocysteine residues, GPX activity could be observed and determined upto 3000 U μmol−1 in the selenium-containing scFv phage abzyme which was isolated by affinity capture against the hapten Be. Also the scFv phage abzymes elicited by different antigens displayed different catalytic activities. After a directed evolution by DNA shuffling to improve the affinity to the hapten Be, a secondary library with GPX activity was created in which the catalytic activity of the selenium-containing scFv phage abzyme could be increased 17%. This study might be helpful for new haptens or antigens design to optimize the abzymes with high binding activities and might also provide a novel scheme for GPX mimic candidates for drug development.
Keyword	scFv abzyme
URL	View the original
Language	英語English
The Source to Article	PB_Publication
PUB ID	37614
Document Type	Journal article
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES
Corresponding Author	Shan, Y
Recommended Citation GB/T 7714	Xu, Y,Li, P,Nie, J,et al. Humanization and directed evolution of the selenium-containing scFv phage abzyme[J]. RSC Advances, 2018, 17218-17223.
APA	Xu, Y., Li, P., Nie, J., Zhao, Q.., Guan, S., Kuai, Z., Qiao, Y., Jiang, X., Li, Y., Li, W., Shi, Y., Kong, W., & Shan, Y (2018). Humanization and directed evolution of the selenium-containing scFv phage abzyme. RSC Advances, 17218-17223.
MLA	Xu, Y,et al."Humanization and directed evolution of the selenium-containing scFv phage abzyme".RSC Advances (2018):17218-17223.

Files in This Item:
There are no files associated with this item.

If you have any objections to this item, please fill out the form below and the administrator will contact you as soon as possible.
Content:
Email：	*
Affiliation No.
Verification Code:	Refresh

Any comments and suggestions are welcomed.
Title:	*
Content:
Email：	*
Verification Code:	Refresh