Effects of insulin on transcriptional response and permeability in an in vitro model of human blood-brain barrier

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Status	已發表Published
	Effects of insulin on transcriptional response and permeability in an in vitro model of human blood-brain barrier
	Kuai, Z; Xu, Y; Zhao, Q.; Liu, J; Guan, S; Qiao, Y; Gong, X; Nie, J; Li, P; Liu, D; Xing, Y; Li, H; Sun, Z; Wang, W; Ning, C; Shi, Y; Kong, W; Shan, Y
	2018
Source Publication	Journal of Cellular Biochemistry
ISSN	0730-2312
Pages	5657-5664
Abstract	Alzheimer's disease (AD) is the most prevalent form of dementia worldwide and is an emerging global epidemic. Active and passive immune therapies targeting beta amyloid (Aβ) have shown very limited evidence in human studies of clinical benefits from these approaches. Epidemiological studies have shown that subjects with type 2 diabetes (T2D) are at higher risk of developing AD. However, whether and how these two conditions are causally linked is unknown. With the purpose of confirming the relationship between T2D and AD, this study specifically focused on effects of insulin in an in vitro model of the human blood‐brain barrier (BBB) and on potential mechanisms of action in the treatment of AD. By using a series of assays to establish a BBB model, we demonstrated that insulin treatment alone could induce the increase of brain endothelial barrier properties. The transcriptional response of hCMEC/D3 cells to activation with different concentrations of insulin was determined by RT‐PCR, and expression levels of genes involved in the control of barrier permeability, including inter‐brain endothelial junctions, integrin‐focal adhesions complexes, and transporter system, were found to be altered by the treatment. Notably, the influence of insulin on expression of the ATP‐binding cassette (ABC) transporter which contributes to the clearance of Aβ was investigated. Insulin up‐regulated adherens junction and tight junction transmembrane proteins, as well as the ABC transporter. By treatment with insulin, the models have major advantages: it is fast, it has low cost, it is fit for considerable samples, and its conditions are under control.
Keyword	human blood‐brain barrier insulin
URL	View the original
Language	英語English
The Source to Article	PB_Publication
PUB ID	37616
Document Type	Journal article
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES
Corresponding Author	Shan, Y
Recommended Citation GB/T 7714	Kuai, Z,Xu, Y,Zhao, Q.,et al. Effects of insulin on transcriptional response and permeability in an in vitro model of human blood-brain barrier[J]. Journal of Cellular Biochemistry, 2018, 5657-5664.
APA	Kuai, Z., Xu, Y., Zhao, Q.., Liu, J., Guan, S., Qiao, Y., Gong, X., Nie, J., Li, P., Liu, D., Xing, Y., Li, H., Sun, Z., Wang, W., Ning, C., Shi, Y., Kong, W., & Shan, Y (2018). Effects of insulin on transcriptional response and permeability in an in vitro model of human blood-brain barrier. Journal of Cellular Biochemistry, 5657-5664.
MLA	Kuai, Z,et al."Effects of insulin on transcriptional response and permeability in an in vitro model of human blood-brain barrier".Journal of Cellular Biochemistry (2018):5657-5664.

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