| Status | 已發表Published |
| Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11 | |
Cheng , M.; Chan, C.; Cheung, R.; Bikkavilli, R.; Zhao, Q.; Au, S.; Chan, P.; Lee, S.; Cheng, G.; Ho, W.; Cheung, W.
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| 2005-12-23 | |
| Source Publication | Biochemical and biophysical research communications
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| ISSN | 0006-291X |
| Pages | 1654-1660 |
| Abstract | Infection of SARS-associated coronavirus (SARS-CoV) induced a strong anti-nucleocapsid (anti-N) antibody response. However, the pathophysiological significance of the anti-N antibodies in SARS pathogenesis is largely unknown. To profile the anti-N antibodies, a phage-displayed scFv library was prepared from mice immunized with heat-inactivated SARS-CoV-infected Vero E6 cell lysate. Specific anti-N scFvs were isolated by panning against a recombinant nucleocapsid protein and reactivity was confirmed with phage-ELISA. Sequence analysis indicated that two of the isolated anti-N scFv clones were identical and displayed a high homology with an scFv specific for interleukin 11 (IL-11), an anti-inflammatory cytokine derived from bone marrow stroma cells. In a neutralization assay, IL-11-induced STAT 3 phosphorylation in rat intestinal epithelial IEC-18 cells was completely suppressed by the anti-N scFv clone L9N01. |
| Keyword | Antibody Interleukin 11 Nucleocapsid protein Phage-display SARS-coronavirus scFv |
| URL | View the original |
| Language | 英語English |
| The Source to Article | PB_Publication |
| PUB ID | 55344 |
| Document Type | Journal article |
| Collection | DEPARTMENT OF BIOMEDICAL SCIENCES |
| Corresponding Author | Cheung, W. |
| Recommended Citation GB/T 7714 | Cheng , M.,Chan, C.,Cheung, R.,et al. Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11[J]. Biochemical and biophysical research communications, 2005, 1654-1660. |
| APA | Cheng , M.., Chan, C.., Cheung, R.., Bikkavilli, R.., Zhao, Q.., Au, S.., Chan, P.., Lee, S.., Cheng, G.., Ho, W.., & Cheung, W. (2005). Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11. Biochemical and biophysical research communications, 1654-1660. |
| MLA | Cheng , M.,et al."Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11".Biochemical and biophysical research communications (2005):1654-1660. |
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