A new format of single chain tri-specific antibody with diminished molecular size efficiently induces ovarian tumor cell killing

UM > Faculty of Health Sciences > DEPARTMENT OF BIOMEDICAL SCIENCES

Status	已發表Published
	A new format of single chain tri-specific antibody with diminished molecular size efficiently induces ovarian tumor cell killing
	Liu, J.; Zhao, Q.; Zhao, B.; Cheng, J.; Wang, X.; Song, L.; Zhang, Z.; Lin, Q.; Huang, H.
	2005-11-01
Source Publication	Biotechnology letters
ISSN	0141-5492
Pages	1821-1827
Abstract	A combination of bi-specific antibodies (BsAb), anti-tumorxanti-CD3 and anti-tumorxanti-CD28, is effective in vitro and in vivo, whereas production of two kinds of bi-specific antibodies is labor intensive and administration is complicated. Accordingly, we previously developed a new model of single chain tri-specific antibody (scTsAb), sTRI, which linked both the CD3 and CD28 signals for T-cell activation in one molecule, and demonstrated its capacity for triggering T-cells to kill ovary tumor cells. To improve the pharmacokinetics further and decrease the immunogenicity of scTsAb, we have now generated a new format of scTsAb, TR3H, whose molecular size is smaller than sTRI. Here we describe the construction, purification and characterization of TR3H. TR3H scTsAb bound to effector cells and tumor target cells specifically and induced redirected lyses of ovary tumor cells through freshly isolated, unstimulated human peripheral blood lymphocytes (PBLs). This new format of scTsAb possesses properties that support its potential as a new tumor immunotherapeutic agent.
Keyword	Human CD3 immunotherapy ovarian cancer single chain tri-specific antibody variable region of heavy chain
URL	View the original
Language	英語English
The Source to Article	PB_Publication
PUB ID	55345
Document Type	Journal article
Collection	DEPARTMENT OF BIOMEDICAL SCIENCES
Corresponding Author	Huang, H.
Recommended Citation GB/T 7714	Liu, J.,Zhao, Q.,Zhao, B.,et al. A new format of single chain tri-specific antibody with diminished molecular size efficiently induces ovarian tumor cell killing[J]. Biotechnology letters, 2005, 1821-1827.
APA	Liu, J.., Zhao, Q.., Zhao, B.., Cheng, J.., Wang, X.., Song, L.., Zhang, Z.., Lin, Q.., & Huang, H. (2005). A new format of single chain tri-specific antibody with diminished molecular size efficiently induces ovarian tumor cell killing. Biotechnology letters, 1821-1827.
MLA	Liu, J.,et al."A new format of single chain tri-specific antibody with diminished molecular size efficiently induces ovarian tumor cell killing".Biotechnology letters (2005):1821-1827.

Files in This Item:
There are no files associated with this item.

If you have any objections to this item, please fill out the form below and the administrator will contact you as soon as possible.
Content:
Email：	*
Affiliation No.
Verification Code:	Refresh

Any comments and suggestions are welcomed.
Title:	*
Content:
Email：	*
Verification Code:	Refresh