葛根芩连汤树脂醇提物可显著缓解伊立替康所致的肠毒性，但其整体质量和临床用药标准有待进一步研究。该文 旨在建立葛根芩连汤树脂醇提物 HPLC 特征图谱，联合网络药理学预测作用靶点和信号通路的方法，预测出药效相关性的核 心化合物，分析预测葛根芩连汤树脂洗脱物缓解伊立替康的潜在的质量标志物( Q-marker) 。根据质量标志物“五原则”的特 有性、可测性和溯源性，结合网络药理学技术可表征葛根芩连汤树脂醇提物的整体功效，初步预测葛根素、小檗碱、黄芩苷、黄 芩素 4 个成分为葛根芩连汤树脂醇提物潜在的 Q-marker，为葛根芩连汤树脂醇提物的质量控制和临床作用机制提供依据和参考。

The resin ethanol extract of Gegen Qinlian Decoction (GGQLD) has been found to significantly alleviate the intestinal toxicity caused by Irinotecan, but further research is needed to establish its overall quality and clinical medication standards. This study aimed to establish an HPLC characteristic fingerprint of the resin ethanol extract of GGQLD, predicted the targets and signaling pathways of its pharmacological effects based on network pharmacology, identified core compounds with pharmacological relevance, and analyzed potential quality markers (Q-markers) of the resin eluate of GGQLD for relieving Irinotecan-induced toxicity. By considering the uniqueness, measurability, and traceability of Q-markers based on the "five principles" of Q-markers and combining them with network pharmacology techniques, the overall efficacy of the resin ethanol extract of GGQLD can be characterized. Preliminary predictions suggested that the four components of puerarin, berberine, baicalin, and baicalein might serve as potential Q-markers for the resin ethanol extract of GGQLD. This study provides a basis and references for the quality control and clinical mechanism of the resin ethanol extract of GGQLD.