Operating reserve, among the most important ancillary services, is a powerful prescription for mitigating increasing uncertainty of renewable generation. Traditional reserve determination neglects uncertainty of renewable generation variations within the dispatch interval, which cannot guarantee sufficient reserve deliverability in real-time operation. This article proposes a probabilistic forecasting-based reserve determination method to efficiently deal with multi-temporal uncertainty of renewable energy in power systems. A novel probabilistic forecasting approach is proposed by integrating Dirichlet process Gaussian mixture model into bootstrap-based extreme learning machine. A unified uncertainty model is constructed for depicting both interval-averaged uncertainty and intra-interval uncertainty by combing the probabilistic forecasts and linearized Itô-process model. In current business practices of many independent system operators, the coordinated determination of two well-designed reserve products, including regulating reserve and ramp capability reserve, is formulated in a two-stage robust optimization framework. A Bernstein polynomial-based model reformulation approach is then employed to handle the existing heterogeneity in the sub-models of each stage. Consequently, the integrated reserve determination is embedded in a generic two-stage robust optimization problem, which can be efficiently solved by the adopted modified column-and-constraint generation method. Finally, numerical simulations are implemented to validate the effectiveness and profitability of the proposed approach.