Manipulation of persistent charges in semiconductor nanostructure is the keypoint to obtain quantum bits towards the application of quantum memoryand information devices. However, realizing persistent charge storage insemiconductor nano-systems is still very challenge due to the disturbancefrom crystal defects and environment conditions. Herein, the two-photonpersistent charging induced long-lasting afterglow and charged excitonformation are observed in CsPbBr3 perovskite nanocrystals (NCs) confined inglass host with effective lifetime surpassing one second, where the glassinclosure provides effective protection. A method combining the femtosecondand second time-resolved transient absorption spectroscopy is explored todetermine the persistent charging possibility of perovskite NCsunambiguously. Meanwhile, with temperature-dependent spectroscopy, theunderlying mechanism of this persistent charging is elucidated. A two-channelcarrier transfer model is proposed involving athermal quantum tunneling andslower thermal-assisted channel. On this basis, two different informationstorage devices are demonstrated with the memory time exceeding two hoursunder low-temperature condition. These results provide a new strategy torealize persistent charging in perovskite NCs and deepen the understandingof the underlying carrier kinetics, which may pave an alternative way towardsnovel information memory and optical data storage applications.