| Residential College | false |
| Status | 已發表Published |
| The regulation of transcriptional activation by NF-κB p52 homodimer and proto-oncogenic Bcl3 | |
Wang, V. Y.-F. 
| |
| 2024-09-02 | |
| Size of Audience | 100 |
| Type of Speaker | Invited Speaker |
| Abstract | The binding of transcription factors (TFs) to their specific DNA response elements in the promoters/enhancers of target genes is the key event regulating gene transcription and consequent cellular events. The NF-κB family of TFs plays a critical role in diverse physiological processes. Our study focuses on the transcriptional regulation by one NF-κB family member, p52, and its specific co-factor, B-cell lymphoma 3 (Bcl3). Bcl3 is an oncoprotein, the constitutive nuclear presence of Bcl3 induces chronic inflammation and proliferation. Bcl3 is also extensively phosphorylated, it associates with NF-κB p52 homodimers to regulate transcription. Using the combination of structural and biochemical studies, we have shown 1) Bcl3 plays an essential role in enhancing p52:p52 homodimer population in cells which is an unique mechanism to p52 within NF-κB family. 2) Crystal structures of p52:p52 homodimer in complex with its natural κB DNA target site(s) revealed a widening of the DNA minor groove compared to all previously known structures of NF-κB-DNA complexes; further MD simulations studies provide new insights into allosteric control by closely related κB DNAs on NF-κB-dependent transcriptional specificity. 3) Phospho-modification mediated changes in Bcl3 regulate DNA accommodation by the Bcl3:(p52:p52) complex. Overall, our studies shed lights on the intricate structural changes driven by both DNA and protein conformation and dynamic states in modulating transcriptional activity. |
| Conference Date | Sept 02-04, 2024 |
| Conference Place | Marburg, Germany |
| Funding Project | Investigation of IκB:NF-κB:DNA Ternary Complex ; Mechanistic Study of NFkappaB and Oncoprotein Bcl3 in Transcriptional Regulation ; Investigation of molecular mechanism of Epstein-Barr virus infection and its translational research |
| Language | 英語English |
| Document Type | Presentation |
| Collection | DEPARTMENT OF BIOMEDICAL SCIENCES |
| Corresponding Author | Wang, V. Y.-F. |
| Affiliation | University of Macau |
| Recommended Citation GB/T 7714 | Wang, V. Y.-F.. The regulation of transcriptional activation by NF-κB p52 homodimer and proto-oncogenic Bcl3, Sept 02-04, 2024. |
| Files in This Item: | Download All | |||||
| File Name/Size | Publications | Version | Access | License | ||
| Book of Abstracts.pd（975KB） | 演讲报告 | 开放获取 | CC BY-NC-SA | View Download | ||
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