| Residential College | false |
| Status | 已發表Published |
| Myricanol prevents aging-related Sarcopenia via targeting peroxiredoxin 5 and Nampt | |
LIN LIGEN 
| |
| 2024 | |
| Size of Audience | 50 |
| Type of Speaker | invited talk |
| Abstract | Sarcopenia is an aging-associated progressive loss of muscle mass and strength, and is accelerated by sedative lifestyle and unhealthy diet. Oxidative damage-induced dysfunctional mitochondria are considered as a culprit of muscle wasting during aging. Herein, we aimed to demonstrate whether myricanol (MY), a cyclic diarylheptanoid derived from Chinese bayberry, protects aged mice against muscle wasting through alleviating oxidative damage, and identify the direct protein target. We discovered that MY protects 18-month-old mice against the loss of muscle mass and strength through scavenging ROS accumulation to rebuild the redox homeostasis and alleviating oxidative damage in mitochondrial DNA to rescue the mitochondrial function. Taking advantage of activity-based protein profiling and biophysical assays including cellular thermal shift assay and isothermal titration calorimetry, peroxiredoxin 5, a critical hydrogen peroxide scavenging enzyme in mitochondria, was discovered and validated as the direct target of MY in protecting C2C12 myotubes against oxidative damage. Through activating peroxiredoxin 5, MY reduced ROS accumulation and damaged mitochondrial DNA. Furthermore, MY selectively binds to the residue Cys100 in peroxiredoxin 5 through hydrophobic interactions and enhances its anti-oxidative activity via the formation of intramolecular disulfide bond. Our findings provide an attractive future direction for therapy against Sarcopenia via peroxiredoxin 5. |
| Source Publication | Acta Materia Medica 杂志创刊两周年暨第二届"我药发现"国际创新药物研发高峰论坛 |
| Conference Place | Beijing, China |
| Document Type | Presentation |
| Collection | Institute of Chinese Medical Sciences |
| Corresponding Author | LIN LIGEN |
| Affiliation | University of Macau |
| First Author Affilication | University of Macau |
| Recommended Citation GB/T 7714 | LIN LIGEN. Myricanol prevents aging-related Sarcopenia via targeting peroxiredoxin 5 and Nampt[Z]. Acta Materia Medica 杂志创刊两周年暨第二届"我药发现"国际创新药物研发高峰论坛 |
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