| Residential College | false |
| Status | 即將出版Forthcoming |
| Establishing a cryopreserved biobank of living tumor tissues for drug sensitivity testing | |
| Chen, Ping1,2,3,4,5; Zhou, Jing Bo1,2,3; Chu, Xiang Peng1,2,3; Feng, Yang Yang1,2,3; Zeng, Qi Bing1,2,3; Lei, Josh Haipeng1,2,3; Wong, Ka Pou1,2,3; Chan, Tai Ip6; Lam, Chon Wa6; Zhu, Wen Li6; Chu, Wai Kuok6; Hu, Feng6; Luo, Guang Hui6; Chan, Kin Iong6; Deng, Chu Xia1,2,3 | |
| 2025 | |
| Source Publication | Bioactive Materials
 |
| ISSN | 2452-199X |
| Abstract | The cryopreservation of cancer tissues to generate frozen libraries is a common practice used worldwide for storing patient samples for later applications. However, frozen samples stored by existing methods cannot be used for initiating living cell cultures, such as patient-derived tumor organoids (PDOs), which offer great potential for personalized treatment. To overcome this challenge, we developed a novel procedure for culturing PDOs using frozen live tumor tissues. We show that tumor specimens stored using this technique maintain their viability and can be successfully used to generate organoids even after long-term freezing, with an impressive success rate of 95.2 %. Importantly, we found that the structural features, tumor marker expression, and drug responses of organoids derived from frozen tissues are similar to those derived from fresh tissues. Moreover, organoids derived from frozen tissues can be routinely passaged and frozen, making them ideal for high-throughput drug screening at any time. Notably, cryopreserved tumor tissues can also be utilized in air-liquid interface (ALI) culture. This method allows for preserving the original tumor microenvironment, making it an invaluable resource for conducting tests on antitumor drug responses, including immune checkpoint inhibitors (ICIs). This innovation has the potential to enable the identification of potentially effective drugs for patients and facilitate the development of novel therapeutic drugs. Thus, we have established protocols for the long-term cryopreservation of cancer tissues to maintain their viability and microenvironment, which are useful for personalized therapy. |
| Keyword | Air-liquid interface (ALI) culture Biobank Living tumor tissues Patient-derived organoids (PDOs) Personalized therapy |
| DOI | 10.1016/j.bioactmat.2024.09.008 |
| URL | View the original |
| Language | 英語English |
| Scopus ID | 2-s2.0-85213496622 |
| Fulltext Access | |
| Citation statistics | |
| Document Type | Journal article |
| Collection | University of Macau |
| Affiliation | 1.Cancer Centre, Faculty of Health Sciences, University of Macau, Macau SAR, 999078, China 2.Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Macau SAR, 999078, China 3.MOE Frontier Science Centre for Precision Oncology, University of Macau, Macau SAR, China 4.Department of Oncology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, 646000, China 5.Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China 6.Kiang Wu Hospital, Macau SAR, 999078, China |
| First Author Affilication | Cancer Centre; Faculty of Health Sciences; University of Macau |
| Recommended Citation GB/T 7714 | Chen, Ping,Zhou, Jing Bo,Chu, Xiang Peng,et al. Establishing a cryopreserved biobank of living tumor tissues for drug sensitivity testing[J]. Bioactive Materials, 2025. |
| APA | Chen, Ping., Zhou, Jing Bo., Chu, Xiang Peng., Feng, Yang Yang., Zeng, Qi Bing., Lei, Josh Haipeng., Wong, Ka Pou., Chan, Tai Ip., Lam, Chon Wa., Zhu, Wen Li., Chu, Wai Kuok., Hu, Feng., Luo, Guang Hui., Chan, Kin Iong., & Deng, Chu Xia (2025). Establishing a cryopreserved biobank of living tumor tissues for drug sensitivity testing. Bioactive Materials. |
| MLA | Chen, Ping,et al."Establishing a cryopreserved biobank of living tumor tissues for drug sensitivity testing".Bioactive Materials (2025). |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment