| Residential College | false |
| Status | 已發表Published |
| Luminescent detection of nicking endonuclease Nb.BsmI activity by using a G-quadruplex-selective iridium(III) complex in aqueous solution | |
Dong, Zhen-Zhen1; Lu, Lihua1,2; Wang, Wanhe1; Li, Guodong3 ; Kang, Tian-Shu3; Han, Quanbin4; Leung, Chung-Hang3  ; Ma, Dik-Lung1,5
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| 2017-07 | |
| Source Publication | SENSORS AND ACTUATORS B-CHEMICAL
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| ISSN | 0925-4005 |
| Volume | 246Pages:826-832 |
| Abstract | A cyclometalated iridium(III) complex has been synthesized as a novel luminescent probe for G-quadruplex (G4) DNA in aqueous solution, and a G4-based assay was employed for the detection of nicking endonuclease activity using Nb.Bsml as a model enzyme. In the assay, an initial hairpin DNA (5'-TG(4)AG(3)TG(4)AG(3)TG(4)A(2)G(3)A(2)TGCTA(3)TAGCAT(2)C(3)T(2)C(4)AC-3') sequence containing the Nb.Bsml recognition site in its stem region and a partially caged G-rich DNA sequence at its 5'-terminus could be cleaved by Nb.BsmI, which liberates the G-rich DNA sequence and enhances the luminescence of complex 1. This assay showed a linear relation between luminescent signal and Nb.Bsml concentration over the range of 0-10 U/mL (R-2=0.9937) with a detection limit for Nb.Bsml of 0.042 U/mL. Furthermore, this assay showed high selectivity for Nb.Bsmi over other nicking endonucleases, and functioned effectively in the presence of cell lysates. (C) 2017 Elsevier B.V. All rights reserved. |
| Keyword | Iridium(Iii) Complex Nicking Endonuclease Luminescence G-quadruplex Probe |
| DOI | 10.1016/j.snb.2017.02.156 |
| URL | View the original |
| Indexed By | SCIE |
| Language | 英語English |
| WOS Research Area | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| WOS Subject | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
| WOS ID | WOS:000400803700101 |
| Publisher | ELSEVIER SCIENCE SA |
| The Source to Article | WOS |
| Scopus ID | 2-s2.0-85014396112 |
| Fulltext Access | |
| Citation statistics | |
| Document Type | Journal article |
| Collection | Institute of Chinese Medical Sciences |
| Corresponding Author | Leung, Chung-Hang; Ma, Dik-Lung |
| Affiliation | 1.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China 2.College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China 3.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China 4.School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China 5.Institute of Research and Continuing Education, Hong Kong Baptist Unversity, Shenzhen 518057, China |
| Corresponding Author Affilication | Institute of Chinese Medical Sciences |
| Recommended Citation GB/T 7714 | Dong, Zhen-Zhen,Lu, Lihua,Wang, Wanhe,et al. Luminescent detection of nicking endonuclease Nb.BsmI activity by using a G-quadruplex-selective iridium(III) complex in aqueous solution[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2017, 246, 826-832. |
| APA | Dong, Zhen-Zhen., Lu, Lihua., Wang, Wanhe., Li, Guodong., Kang, Tian-Shu., Han, Quanbin., Leung, Chung-Hang., & Ma, Dik-Lung (2017). Luminescent detection of nicking endonuclease Nb.BsmI activity by using a G-quadruplex-selective iridium(III) complex in aqueous solution. SENSORS AND ACTUATORS B-CHEMICAL, 246, 826-832. |
| MLA | Dong, Zhen-Zhen,et al."Luminescent detection of nicking endonuclease Nb.BsmI activity by using a G-quadruplex-selective iridium(III) complex in aqueous solution".SENSORS AND ACTUATORS B-CHEMICAL 246(2017):826-832. |
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