Phytochemical investigation of the roots of Dianella ensifolia led to the isolation of eleven known compounds, including two aromatics (1, 2), two chromones (3, 4), and seven flavonoids (5-11). The structures of these compounds were elucidated on the basis of spectral analysis and comparison with data reported in literature. In the present research, all of the isolated compounds 1-11 were reported for the first time in the species D. ensifolia. Compounds 1-4 and 7-11 were firstly isolated from the genus Dianella. Compounds 1, 2, 8, and 9 have not been reported from any species in Liliaceae family. The chemotaxonomic significance of the isolated compounds was summarized.