Objective: To investigate the nonvolatile chemical constituents from the ethanol extract of the stems of Pogostemon cablin. Method: The constituents were isolated and purified by repeated column chromatography on silica gel and Sephadex LH-20. Their structures were identified by physicochemical properties and spectroscopic analysis. Result: Twelve compounds were isolated and identified as tilianin(1), diosmetin-7-O-β-D-glucopyranoside(2), 3‴-O-methylcrenatoside(3), uracil(4), soya-cerebroside I and II(5), agastachoside(6), apigenin-7-O-(3″, 6″-(E)-p-coumaroyl)-β-D- galactopyranoside(7), 5-hydroxy-3, 3′,4′,7-tetramethoxy flavone(8), 4′,5-dihydroxy-3,3′,7-trimethoxyflavone(9), acacetin(10), crenatoside(11), isocrenatoside(12). Conclusion: Compounds 1, 2, 4-7, 10 were isolated from the genus Pogostemon for the first time.