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Naked-eye detection of polymerase chain reaction on microfluidics
Shen,Ren1,2; Jia,Yanwei1,2,3; Mak,Pui In1,2; Martins,Rui P.1,2,4
2020-10
Conference NameThe 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Source PublicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Pages1097-1098
Conference Date2020/10/04-2020/10/09
Conference Placeonline
Abstract

In this study, we established a visual detection method of polymerase chain reaction (PCR) products on digital microfluidic chips. Using a recently established DNA amplification enhancer, we were able to apply the dsDNA-binding dyes in PCR at a remarkably high concentration and lifted the dyes' signal with positive samples. Excited with a UV pen or the mobile phone flashlight, the amplification results of on-chip PCR were easily visualized by naked eyes.

KeywordMicrofluidics Naked Eye Detection Pcr
URLView the original
Language英語English
Scopus ID2-s2.0-85098240360
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Document TypeConference paper
CollectionFaculty of Health Sciences
Faculty of Science and Technology
THE STATE KEY LABORATORY OF ANALOG AND MIXED-SIGNAL VLSI (UNIVERSITY OF MACAU)
INSTITUTE OF MICROELECTRONICS
DEPARTMENT OF ELECTRICAL AND COMPUTER ENGINEERING
DEPARTMENT OF BIOMEDICAL SCIENCES
Corresponding AuthorJia,Yanwei
Affiliation1.State-Key Laboratory of Analog and Mixed-Signal,VLSI,Institute of Microelectronics,University of Macau,Macao
2.Faculty of Science and Technology,ECE,University of Macau,Macao
3.Faculty of Health Sciences,University of Macau,Macao
4.Instituto Superior Técnico,Universidade de Lisboa,Portugal
First Author AffilicationUniversity of Macau;  Faculty of Science and Technology
Corresponding Author AffilicationUniversity of Macau;  Faculty of Science and Technology;  Faculty of Health Sciences
Recommended Citation
GB/T 7714
Shen,Ren,Jia,Yanwei,Mak,Pui In,et al. Naked-eye detection of polymerase chain reaction on microfluidics[C], 2020, 1097-1098.
APA Shen,Ren., Jia,Yanwei., Mak,Pui In., & Martins,Rui P. (2020). Naked-eye detection of polymerase chain reaction on microfluidics. MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 1097-1098.
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