Pressurized liquid extraction (PLE) and capillary electrochromatography(CEC) methods were developed for the simultaneous determination of five flavonoids, namely liquiritin, isoliquiritin, ononin, liquiriti-genin and isoliquiritigenin, in licorice using baicalein as internal standard (IS). Peak suppression technique was used for the quantification of ononin because of its poor resolution with isoliquiritin. The analysis was performed on a Hypersil C₁₈ capillary (3 μm, 100μm/25cm) with a mixture of10mM phosphate buffer (pH 3.0)/ACN (65:35, v/v) as mobile phase running at 25kV and 30°C. The detection wavelengths were set at 275 nm (without reference wavelength for liquiritin and liquiritigenin), 360 nm (without reference wavelength for isoliquiritin and isoliquiritigenin) and 254nm (withreference wavelength of405nmfor ononin). All calibration curves showed good linearity (R² >0.9993) within the test ranges. The LOD and LOQ were lower than 2.1 and 8.3 μg/mL, respectively. The RSDs of intra- and interday for relative peak areas of five analytes to IS were less than 3.8 and 4.7%, respectively, and the recoveries were 98.2-103.8%. The validated method was successfully applied to the quantitative analysis of five flavonoids in licorice, which is helpful to its quality control.