Chiral ligand conjugated transition metal oxide nanoparticles (NPs) are a promising platform for chiral recognition, biochemical sensing, and chiroptics. Herein, we present chirality-based strategy for effective sensing of mercury ions via ligand-induced chirality derived from metal-to-ligand charge transfer (MLCT) effects. The ligand competition effect between molybdenum and heavy metal ions such as mercury is designated to be essential for MLCT chirality. With this know-how, mercury ions, which have a larger stability constant (K ) than molybdenum, can be selectively identified and quantified with a limit of detection (LOD) of 0.08 and 0.12 nmol/L for D-cysteine and L-cysteine (Cys) capped MoO NPs. Such chiral chemical sensing nanosystems would be an ideal prototype for biochemical sensing with a significant impact on the field of biosensing, biological systems, and water research-based nanotoxicology.