Plasmonic sensing relies on the interaction between the electromagneticlightwaveandfreeelectronsthatresideattheinterfaceoftwomaterials,one of them being a metal. Surface plasmon resonance (SPR)-based biosensinghas distinct advantages of providingreal-time and label-free detectiontechnique that is capable of giving accurate and repeatable results. 2Dnanomaterials (graphene and transition metal dichalcogenides [TMDCs]such as MoS2and WS2) have recently gained high popularity in thefield ofbiomedical sciences. Using Biacore commercial SPR machine manufactured byGE Healthcare, the efficacy of various combinations of these materials coatedon the plasmonic metal gold (Au) (AuþMoS2,AuþWS2,Auþgraphene,AuþgrapheneþMoS2,andAuþgrapheneþWS2) is evaluated, in enhancingthe sensitivity of the biosensor as compared with the standard Kretschmannconfiguration (only Au). Some simulation results are also presented at incidentlight wavelengths of 633 and 785 nm that are based on the angular interro-gation method. To conclude, it is found that the sensitivity in the increasingorder is: only Au