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Point-of-care testing detection methods for COVID-19
Song, Qi1,2; Sun, Xindi3; Dai, Ziyi4; Gao, Yibo5; Gong, Xiuqing3; Zhou, Bingpu4; Wu, Jinbo3; Wen, Weijia1
Source PublicationLab on a Chip
ISSN1473-0197
2021-05-07
Abstract

COVID-19 is an acute respiratory disease caused by SARS-CoV-2, which has high transmissibility. People infected with SARS-CoV-2 can develop symptoms including cough, fever, pneumonia and other complications, which in severe cases could lead to death. In addition, a proportion of people infected with SARS-CoV-2 may be asymptomatic. At present, the primary diagnostic method for COVID-19 is reverse transcription-polymerase chain reaction (RT-PCR), which tests patient samples including nasopharyngeal swabs, sputum and other lower respiratory tract secretions. Other detection methods, e.g., isothermal nucleic acid amplification, CRISPR, immunochromatography, enzyme-linked immunosorbent assay (ELISA) and electrochemical sensors are also in use. As the current testing methods are mostly performed at central hospitals and third-party testing centres, the testing systems used mostly employ large, high-throughput, automated equipment. Given the current situation of the epidemic, point-of-care testing (POCT) is advantageous in terms of its ease of use, greater approachability on the user's end, more timely detection, and comparable accuracy and sensitivity, which could reduce the testing load on central hospitals. POCT is thus conducive to daily epidemic control and achieving early detection and treatment. This paper summarises the latest research advances in POCT-based SARS-CoV-2 detection methods, compares three categories of commercially available products, i.e., nucleic acid tests, immunoassays and novel sensors, and proposes the expectations for the development of POCT-based SARS-CoV-2 detection including greater accessibility, higher sensitivity and lower costs. 

Language英語English
DOI10.1039/d0lc01156h
URLView the original
Volume21
Issue9
Pages1634-1660
Scopus ID2-s2.0-85104994154
Fulltext Access
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Document TypeReview article
CollectionINSTITUTE OF APPLIED PHYSICS AND MATERIALS ENGINEERING
Corresponding AuthorZhou, Bingpu; Wu, Jinbo; Wen, Weijia
Affiliation1.Department of Physics, Hong Kong University of Science and Technology, Kowloon, Clear Water Bay, Hong Kong
2.Guangzhou HKUST, Fok Ying Tung Research Institute, Guangzhou Guangdong, China
3.Materials Genome Institute, Shanghai University, Shanghai, China
4.Joint Key Laboratory of the Ministry of Education, Institute of Applied Physics and Materials Engineering, University of Macau, Macao
5.Shenzhen Shineway Technology Corporation, Shenzhen Guangdong, China
Corresponding Author AffilicationINSTITUTE OF APPLIED PHYSICS AND MATERIALS ENGINEERING
Recommended Citation
GB/T 7714
Song, Qi,Sun, Xindi,Dai, Ziyi,et al. Point-of-care testing detection methods for COVID-19[J]. Lab on a Chip, 2021, 21(9), 1634-1660.
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