| Residential College | false |
| Status | 已發表Published |
| Development of an Aptamer-Based Sensing Platform for Metal Ions, Proteins, and Small Molecules through Terminal Deoxynucleotidyl Transferase Induced G-Quadruplex Formation | |
Leung K.-H.1; He B.1; Yang C.2 ; Leung C.-H.2  ; Wang H.-M.D.3; Ma D.-L.1
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| 2015 | |
| Source Publication | ACS Applied Materials and Interfaces
 |
| ISSN | 19448244 |
| Volume | 7Issue:43Pages:24046–24052 |
| Abstract | We report a label-free, structure-independent luminescent-sensing platform for metal ions, proteins, and small molecules utilizing an Ir(III) complex, terminal deoxynucleotidyl transferase (TdT), and a structure-folding aptamer. A novel G-quadruplex-selective Ir(III) complex was identified to detect the nascent G-quadruplex motifs with an enhanced luminescence response. Unlike most label-free DNA-based assays reported in the literature, this sensing platform does not require a specific secondary structure of aptamer, thus greatly simplifying DNA design. The detection platform was demonstrated by the detection of K+ ions, thrombin, and cocaine as representative examples of metal ions, proteins, and small molecules. © 2015 American Chemical Society. |
| Keyword | G-quadruplex Iridium(Iii) Complex Label-free Tdt Universal Detection |
| DOI | 10.1021/acsami.5b08314 |
| URL | View the original |
| Indexed By | SCIE |
| Language | 英語English |
| WOS Research Area | Science & Technology - Other Topics ; Materials Science |
| WOS Subject | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| WOS ID | WOS:000364355500027 |
| The Source to Article | Scopus |
| Scopus ID | 2-s2.0-84946607011 |
| Fulltext Access | |
| Citation statistics | |
| Document Type | Journal article |
| Collection | Institute of Chinese Medical Sciences |
| Corresponding Author | Leung C.-H.; Wang H.-M.D.; Ma D.-L. |
| Affiliation | 1.Department of Chemistry, Partner State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China 2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China 3.Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan 4.Partner State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China |
| Corresponding Author Affilication | Institute of Chinese Medical Sciences |
| Recommended Citation GB/T 7714 | Leung K.-H.,He B.,Yang C.,et al. Development of an Aptamer-Based Sensing Platform for Metal Ions, Proteins, and Small Molecules through Terminal Deoxynucleotidyl Transferase Induced G-Quadruplex Formation[J]. ACS Applied Materials and Interfaces, 2015, 7(43), 24046–24052. |
| APA | Leung K.-H.., He B.., Yang C.., Leung C.-H.., Wang H.-M.D.., & Ma D.-L. (2015). Development of an Aptamer-Based Sensing Platform for Metal Ions, Proteins, and Small Molecules through Terminal Deoxynucleotidyl Transferase Induced G-Quadruplex Formation. ACS Applied Materials and Interfaces, 7(43), 24046–24052. |
| MLA | Leung K.-H.,et al."Development of an Aptamer-Based Sensing Platform for Metal Ions, Proteins, and Small Molecules through Terminal Deoxynucleotidyl Transferase Induced G-Quadruplex Formation".ACS Applied Materials and Interfaces 7.43(2015):24046–24052. |
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