Lipid productivity by mixed culture of Rhodosporidium toruloides and Chlorella pyrenoidosa was studied using 1:1 mixed real wastewater from distillery and local municipal wastewater treatment plant with initial soluble chemical oxygen demand (SCOD) around 25,000mg/L, initial cell density of 2×10⁷cells/mL (yeast) and 5×10⁶cells/mL (microalga), at 30°C and 2.93W/m² (2000lux, 12:12h light and dark cycles). Lipid content and lipid yield achieved were 63.45±2.58% and 4.60±0.36g/L with the associated removal efficiencies for SCOD, total nitrogen (TN), and total phosphorus (TP) at 95.34±0.07%, 51.18±2.17%, and 89.29±4.91%, respectively, after 5days of cultivation without the pH adjustment. Inoculation of microalgae at 40h of the initial yeast cultivation and harvesting part of inactive biomass at 72h by sedimentation could improve both lipid production and wastewater treatment efficiency under non-sterile conditions.