The current study aimed to evaluate the reliability and validity of the Chinese version of the EmQue-CA, as well as the measurement invariance across gender. A sample of 1107 students (498 boys and 609 girls) enrolled in fourth grade to twelfth grade (M = 13.29; SD = 2.22) participated in the current study. Results indicated that the Chinese version had a similar three-factor model with two items deleted from the original form. The reliability, including internal consistency and test-retest reliability, of the Chinese version of the EmQue-CA was acceptable. Concurrent validity was assessed by examining the correlations of the EmQue-CA with the Basic Empathy Scale and the prosocial behavior subscale of the Strengths and Difficulties Questionnaire, with significant associations delivered. The results of convergent validity demonstrated strong correlations of the EmQue-CA subscales with callous unemotional traits and bullying. For the measurement invariance across gender, configural invariance and metric invariance were supported by the data, while scalar invariance failed. Overall, the EmQue-CA is a valuable tool for assessing empathy in Chinese children and adolescents.