The COVID-19 pandemic imposes an increasing demand for service robots as a substitute for humans to conduct various types of work in contaminated areas. Such work includes logistics, patient care, and disinfection, which can reduce the risk of human exposure to the highly contagious and deadly virus. This paper presents the design and development of Smart Cleaner, which is a new cost-effective autonomous indoor disinfection robot. It integrates a wheeled mobile robot platform and a hydrogen peroxide atomization device for automated disinfection operation in the complex indoor environment. Through the system integration of various hardware components and software programming, a prototype of the disinfection robot has been fabricated for experimental investigation. A simulation study of the drymist hydrogen peroxide disinfection model was carried out to understand the diffusion of disinfectant in a room environment. The effectiveness of the developed robot was verified in practical scenarios, such as hospital, hotel, office, and laboratory. The effect of disinfection was validated by a qualified third-party testing agency. Results demonstrate the high efficiency of the developed disinfection robot dedicated to autonomous indoor disinfection work.