Residential College | true |
Status | 已發表Published |
Polyphyllin VII induces apoptosis in HepG2 cells through ROS-mediated mitochondrial dysfunction and MAPK pathways | |
Zhang C.1; Jia X.1; Bao J.1; Chen S.1; Wang K.1; Zhang Y.1; Li P.1; Wan J.-B.1; Su H.1; Wang Y.1; Mei Z.2; He C.1 | |
2016 | |
Source Publication | BMC complementary and alternative medicine |
Volume | 16Pages:58 |
Abstract | BACKGROUND: Paris polyphylla is an oriental folk medicine that has anticancer activities both in vivo and in vitro. Polyphyllin VII (PP7), a pennogenyl saponin from P. polyphylla has been found to exert strong anticancer activity. However, the underlying mechanisms are poorly understood. In the present study, the anticancer effect of polyphyllin VII against human liver cancer cells and the molecular mechanisms were investigated.METHODS: Cellular viability was measured by MTT assay. Apoptosis, intracellular reactive oxygen species (ROS) and mitochondrial membrane potential levels were evaluated using the InCell 2000 confocal microscope. The expression levels of apoptotic-related proteins were evaluated by Western blotting.RESULTS: PP7 strongly inhibited the cell growth and induced apoptosis and necrosis in hepatocellular carcinoma HepG2 cells. Meanwhile, PP7 up-regulated the levels of Bax/Bcl-2, cytochrome c, the cleaved forms of caspases-3, -8, -9, and poly (ADP-ribose) polymerase in a dose- and time-dependent manner, indicating that PP7 induced apoptosis in HepG2 cells through both intrinsic and extrinsic pathways. Moreover, PP7 provoked the production of intracellular ROS and the depolarization of mitochondrial membrane potential. Further analysis showed that PP7 significantly augmented the phosphorylation of JNK, ERK and p38, the major components of mitogen-activated protein kinase (MAPK) pathways, and the expressions of tumor suppressor proteins p53 and PTEN. In addition, PP7-induced apoptosis was remarkably attenuated by MAPK inhibitors and ROS inhibitor.CONCLUSIONS: These results demonstrated that PP7 induced apoptotic cell death in HepG2 cells through both intrinsic and extrinsic pathways by promoting the generation of mitochondrial-mediated ROS and activating MAPK and PTEN/p53 pathways. |
Keyword | Polyphyllin Vii Apoptosis Mapk Pathways Anticancer Hepg2 Cells |
DOI | 10.1186/s12906-016-1036-x |
URL | View the original |
Indexed By | SCIE |
WOS Research Area | Integrative & Complementary Medicine |
WOS Subject | Integrative & Complementary Medicine |
WOS ID | WOS:000369606200001 |
The Source to Article | Scopus |
Scopus ID | 2-s2.0-84976589112 |
Fulltext Access | |
Citation statistics | |
Document Type | Journal article |
Collection | Institute of Chinese Medical Sciences |
Corresponding Author | He C. |
Affiliation | 1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, N22-7038, Avenida da Universidade, Taipa, Macao, 999078, China 2.College of Pharmacy, South Central University for Nationalities, Wuhan, 430074, China |
First Author Affilication | Institute of Chinese Medical Sciences |
Corresponding Author Affilication | Institute of Chinese Medical Sciences |
Recommended Citation GB/T 7714 | Zhang C.,Jia X.,Bao J.,et al. Polyphyllin VII induces apoptosis in HepG2 cells through ROS-mediated mitochondrial dysfunction and MAPK pathways[J]. BMC complementary and alternative medicine, 2016, 16, 58. |
APA | Zhang C.., Jia X.., Bao J.., Chen S.., Wang K.., Zhang Y.., Li P.., Wan J.-B.., Su H.., Wang Y.., Mei Z.., & He C. (2016). Polyphyllin VII induces apoptosis in HepG2 cells through ROS-mediated mitochondrial dysfunction and MAPK pathways. BMC complementary and alternative medicine, 16, 58. |
MLA | Zhang C.,et al."Polyphyllin VII induces apoptosis in HepG2 cells through ROS-mediated mitochondrial dysfunction and MAPK pathways".BMC complementary and alternative medicine 16(2016):58. |
Files in This Item: | There are no files associated with this item. |
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment