Objective: To study the chemical constituents in the roots of Pueraria lobata. Method: Various chromatographic methods were employed to separate of chemical constituents and the spectroscopic methods were used to elucidate the structure. Result: Twenty-two compounds were isolated and characterized as β-sitosterol palmitate (1), β-sitosterol (2), lupeol (3), lupeone (4), puerarol (5), diisobutyl phthalate (6), bis(2-ethylhexyl) phthalate (7), sophoracoumestan A (8), coumestrol (9) , allantion (10), dadzein (11), formononetin (12), 3′-methoxy daidzein (13), ononin (14), 3′-hydroxy dadzein (15) , genistin (16), dadzin (17), 8-methoxy ononin (18), sissotorin (19), (-)-puerol B 2-O-glucopyranoside (20), (6S, 9R)-roseoside (21) and sucrose (22), respectively. Conclusion: Compounds 1, 5-8, 15, 18, 21 and 22 were isolated from P. lobata for the first time, and componds 1, 5-7, 15, 18, 21 and 22 were isolated from the genus Pueraria for the first time.