Isoflavonoids in Pueraria lobata were extracted by pressurized solvent extraction (PSE). Orthogonal design was used to examine the factor that affects theefficiency of PSE extraction, and the quantitative marker was the total content of Puerarin, Daidzin and Daizein which were determined simultaneously by high performance liquid chromatography. The optimum conditions of P,SE extraction of isoflavonoids from Pueraria lobata were as follows: methanol as solvent, temperature at 140 degrees C, pressure under 8.27 MPa, 10 min duration and transpositional volume as 60%. Furthermore, the yields ofisoflavonoids from Pueraria lobata by using PSE and extraction method according to the Chinese Pharmacopoeia (ChP) monography was compared. PSE technology has the advantages of shortening the extraction time, decreasing the consumption of solvent, increasing extraction efficiency and automatic operating capabilities.